Free Fatty Acid Assay Kit, BioAssay™

Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome.

F6180-50: Free Fatty Acid Assay Kit, BioAssay™ method provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl- CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585nm) is directly proportional to the free fatty acid concentration in the sample.

F6180-50A: Assay Buffer, 1x20ml
F6180-50B: CoSubstrate, 1x120ul
F6180-50C: Dye Reagent, 1x120ul
F6180-50D: Enzyme A, 1x120ul
F6180-50E: Enzyme B, 1x120ul
F6180-50F: Standard (1mM palmitic acid), 1x1ml

Storage and Stability:
Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.