FST ELISA Kit (Chicken)
Référence OKEH03840
Conditionnement : 96T
Marque : Aviva Systems Biology
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FST ELISA Kit (Chicken) (OKEH03840)
| Datasheets/Manuals | Printable datasheet for FST ELISA Kit (Chicken) (OKEH03840) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology FST ELISA Kit (Chicken) (OKEH03840) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FST has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FST is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FST captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 38 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Follistatin | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | FST |
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| Gene Full Name | follistatin |
| Alias Symbols | activin-binding protein, follistatin, FS. |
| NCBI Gene Id | 396119 |
| Protein Name | Follistatin |
| Description of Target | Binds directly to activin and functions as an activin antagonist. Inhibits activin A signaling in the iris and regulates somatostatin phenotype in ciliary ganglion neurons. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). |
| Uniprot ID | Q90844 |
| Protein Accession # | NP_990531.1 |
| Nucleotide Accession # | NM_205200.1 |
| Protein Size (# AA) | 343 |
| Molecular Weight | 38 kDa |
