Biovalley > GAPDH Antibody - HRP Conjugated
GAPDH Antibody - HRP Conjugated
Référence OACA00439
Conditionnement : 100ug
Marque : Aviva Systems Biology
GAPDH Antibody - HRP Conjugated (OACA00439)
| Datasheets/Manuals | Printable datasheet for GAPDH Antibody - HRP Conjugated (OACA00439) (OACA00439) |
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| Predicted Species Reactivity | Chicken |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | HRP |
| Application | ELISA |
| Reconstitution and Storage | -20°C or -80°C |
| Immunogen | Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA) |
| Purification | Protein G Purified |
| Purity | Caprylic Acid Ammonium Sulfate Precipitation purified |
| Storage Buffer | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reference | [1] Complete nucleotide sequence of the messenger RNA coding for chicken muscle glyceraldehyde-3-phosphate dehydrogenase.'Panabieres F., Piechaczyk M., Rainer B., Dani C., Fort P., Riaad S., Marty L., Imbach J.L., Jeanteur P., Blanchard J.-M.Biochem. Biophys. Res. Commun. 118:767-773(1984) [2] Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle.'Dugaiczyk A., Haron J.A., Stone E.M., Dennison O.E., Rothblum K.N., Schwartz R.J.Biochemistry 22:1605-1613(1983) [3] Complete sequence of the chicken glyceraldehyde-3-phosphate dehydrogenase gene.'Stone E.M., Rothblum K.N., Alevy M.C., Kuo T.M., Schwartz R.J.Proc. Natl. Acad. Sci. U.S.A. 82:1628-1632(1985) |
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| Gene Symbol | GAPDH |
| Gene Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Alias Symbols | glyceraldehyde-3-phosphate dehydrogenase;KNC-NDS6;peptidyl-cysteine S-nitrosylase GAPDH. |
| NCBI Gene Id | 374193 |
| Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
| Description of Target | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins (By similarity). |
| Uniprot ID | P00356 |
| Protein Accession # | NP_001243728.1; NP_001276674.1; NP_001276675.1; NP_002037.2 |
| Nucleotide Accession # | NM_001256799.2; NM_001289745.1; NM_001289746.1; NM_002046.5 |
Protein interactions
| Name | # of Products |
|---|---|
| ACTC1 | 82 |
| ANXA1 | 108 |
| ANXA7 | 270 |
| ARL15 | 53 |
| BID | 117 |
| BTBD2 | 61 |
| CAMK2B | 59 |
| CBL | 471 |
| CCNB1 | 173 |
| CD4 | 497 |
| CDKN1A | 474 |
| CDKN2A | 229 |
| GADD45A | 158 |
| GAS7 | 76 |
| GOT2 | 54 |
| GSK3B | 566 |
| HES1 | 107 |
| HNF4G | 64 |
| HTT | 289 |
| KARS | 73 |
| KCNE3 | 53 |
| MAPK1 | 575 |
| Mapk13 | 300 |
| NFYC | 72 |
| NR1H4 | 63 |
| OSMR | 41 |
| OSTF1 | 39 |
| PCNA | 402 |
| POU5F1 | 224 |
| PPM1E | 30 |
| PPP2R2A | 114 |
| PRPF40A | 218 |
| PSMA3 | 438 |
| RBM5 | 36 |
| SET | 124 |
| SIRT1 | 310 |
| SMN1 | 388 |
| SNCA | 228 |
| SUMO1 | 972 |
| TK1 | 389 |
| TPPP | 33 |
| UBC | 7030 |
| USP25 | 66 |
| YWHAE | 312 |
| YWHAQ | 716 |
Biological pathways
| Name | # of Products |
|---|---|
| Gluconeogenesis | 156 |
| Glycolysis | 159 |
| Neuron apoptosis | 73 |
| Peptidyl-cysteine S-trans-nitrosylation | 32 |
| Protein stabilization | 177 |
Biological process
| Name | # of Products |
|---|---|
| Carbohydrate metabolic process | 247 |
| Gluconeogenesis | 50 |
| Glucose metabolic process | 107 |
| Glycolysis | 53 |
| Neuron apoptosis | 25 |
| Peptidyl-cysteine S-trans-nitrosylation | 1 |
| Protein stabilization | 64 |
| Small molecule metabolic process | 825 |
Cellular components
| Name | # of Products |
|---|---|
| Cytoplasm | 4549 |
| Cytosol | 1868 |
| Membrane | 2769 |
| Nucleus | 4914 |
| Perinuclear region of cytoplasm | 371 |
Protein function
| Name | # of Products |
|---|---|
| Glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity | 32 |
| NAD binding | 118 |
| NADP binding | 101 |
| Oxidoreductase activity | 982 |
| Peptidyl-cysteine S-nitrosylase activity | 30 |
| Protein binding | 12191 |
| Transferase activity | 1042 |
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