Glucose oxidase is used in the enzymatic determination of D-glucose in solution (1). Glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide. Horseradish peroxidase is then used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
Ca. 300 U/mg protein.

Unit definition: 1 U catalyzes the oxidation of 1 µmole glucose to glucuronic acid per minute at 25 °C, pH 7 coupled with peroxidase and o-dianisidine (1).
Extraneous activities: Amylase, saccharase and maltase less than 0.05 %; GOD/catalase min. 2000.


References:

1. Kunst, A. et al. (1984) Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.) 3rd Ed. Vol. 6, p. 178-85
2. Tsuge, H. & Mitsuda, H. (1973) J. Biochem. (Tokyo) 73, 199-206
3. Pazur, J.H. (1966) in Methods in Enzymology (Colowick, S.P. & Kaplan, N.O., eds.) Vol. IX, 82-7
4. O'Malley, J.J. & Weaver, J.L. (1972) Biochemistry 11, 3527-32

DANGER

Hazard Statements	H334
Precautions	P261 - P285
Reaction	P304 +P341 - P342 +P311

EINECS: 232-601-0 • WGK: 1L • HS: 35079090
Storage Temperature: -15 °C to -25 °C