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> Glutathione Reductase Assay Kit

Glutathione Reductase Assay Kit

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Référence MBS480419-5x1

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Glutathione Reductase (GSR) Assay Kit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS480419

Principle of the Assay: This assay is based on the oxidation of NADPH to NADP+ catalyzed by a limiting concentration of glutathione reductase. One GR activity unit is defined as the amount of enzyme catalyzing the reduction of one micromole of GSSG per minute… More details

Product Overview

Product Name
Glutathione Reductase (GSR), Assay Kit
Full Product Name
Glutathione Reductase Assay Kit
Product Gene Name
GSR assay kit
Product Synonym Names
Spectrophotometric Assay for Glutathione Reductase
Preparation and Storage
4 degree C
Molecular Weight
25,081 Da
3D Structure
ModBase 3D Structure for Q03504

Typical Testing Data/Standard Curve (for reference only) of GSR assay kit

GSR Assay Kit – Cat# MBS480419

Product References and Citations for GSR assay kit

1. Dolphin D, Poulson R, Avramovic O, Eds. (1989) Glutathione: Chemical, Biochemical and Metabolic Aspects, Vols. A and B, J. Wiley and Sons.
2. Bashir A, Perham RN, Scrutton NS, Berry A (1995) "Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase" Biochem.J. 312, 527-533.
3. Massey V, Williams CH (1965) "On the mechanism of yeast glutathione reductase" J.Biol.Chem. 240 (11), 4470-4480.
4. Worthington DJ, Rosemeyer MA (1976) "Glutathione reductase from human erythrocytes. Catalytic properties and aggregation" Eur.J.Biochem. 67, 231-238.
5. García-Alfonso C, Martìnez-Galisteo, Llobell A, Bárcena JA, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (1), 61-68.
6. Carlberg I, Mannervik B (1985) "Glutathione reductase" Meth.Enzymol. 113, 485-490.
7. Peindo J, García-Alfonso C, Martínez-Galisteo E, Llobell A, López-Barea J (1991) "Metals are directly involved in the redox interconversion of Saccharomyces cervisiae glutathione reductase" Molec.Cell.Biol. 101, 175-187.
8. García-Alfonso C, Martínez-Galisteo E, Llobell A, Bácena J, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (4), 513-520.
9. Smith I, Vierheller T, Thorne C (1988) "Assay of glutathione reductase in crude tissue homogenates using 5,5'- dithiobis(2-nitrobenzoic acid)" Anal.Biochem. 75, 408-413.
10. Beutler, E (1969) "Effect of flavin compounds on the glutathione reductase activity: in vitro and in vivo studies" J.Clin.Invest. 48, 1957-1966.

Other Notes

Small volumes of GSR assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GSR purchase

MBS480419 is an assay kit targeting GSR. Also known as Glutathione Reductase Assay Kit.

Additional Details

NCBI/Uniprot data below describe general gene information for GSR. It may not necessarily be applicable to this product.

NCBI GI #
31829
NCBI GeneID
2936
NCBI Accession #
CAA38367.1
UniProt Primary Accession #
Q03504
UniProt Related Accession #
P00390
NCBI Official Full Name
glutathione reductase, partial
NCBI Official Synonym Full Names
glutathione reductase
NCBI Official Symbol
GSR
NCBI Official Synonym Symbols
HEL-75
UniProt Protein Name
Glutathione reductase
UniProt Synonym Protein Names
Glutathione reductase
Protein Family
Glutathione reductase
UniProt Entry Name
Q03504_HUMAN
NCBI Protein Information
glutathione reductase, mitochondrial; GR; GRase; epididymis luminal protein 75

NCBI Summary for GSR

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]
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UniProt Comments for GSR

GSR: a class-I pyridine nucleotide-disulfide oxidoreductase. Maintains high levels of reduced glutathione in the cytosol. Alternative initiation results in a mitochondrial and a cytoplasmic isoform.

Protein type: Oxidoreductase; EC 1.8.1.7; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: mitochondrial matrix; cytosol; external side of plasma membrane

Molecular Function: mercury (II) reductase activity; electron carrier activity; FAD binding; mercury ion binding; glutathione-disulfide reductase activity; NADP binding

Biological Process: response to reactive oxygen species; glutathione metabolic process; nucleobase, nucleoside and nucleotide metabolic process; cell redox homeostasis; nucleobase, nucleoside and nucleotide interconversion; detoxification of mercury ion
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Research Articles on GSR

1. GSR was the most significant single SNP association with systemic lupus erythematosus in African Americans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GSR assay kit

 Reference Product  PubMed Publications
 CAT assay kit  >49 publications with GSR and CAT
 TXN assay kit  >11 publications with GSR and TXN
 GSS assay kit  >9 publications with GSR and GSS
 GSTP1 assay kit  >6 publications with GSR and GSTP1
 GSTA1 assay kit  >3 publications with GSR and GSTA1
 GSTM1 assay kit  >3 publications with GSR and GSTM1
 GGT1 assay kit  >2 publications with GSR and GGT1
 GSTO1 assay kit  >1 publications with GSR and GSTO1

Pathways associated with GSR assay kit

 Products by Pathway  Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram

Organs/Tissues associated with GSR assay kit

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >458 publications with GSR and Skin
 Blood Antibodies  >146 publications with GSR and Blood
 Heart Antibodies  >102 publications with GSR and Heart
 Brain Antibodies  >73 publications with GSR and Brain
 Liver Antibodies  >37 publications with GSR and Liver
 Kidney Antibodies  >28 publications with GSR and Kidney
 Vascular Antibodies  >22 publications with GSR and Vascular
 Nerve Antibodies  >20 publications with GSR and Nerve
 Eye Antibodies  >20 publications with GSR and Eye
 Lung Antibodies  >16 publications with GSR and Lung