Glycogen Synthase Kinase 3 beta (GSK3b)

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3α and GSK-3β) share 85% amino acid identity. GSK-3β, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Hybridoma:
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human Glycogen Synthase Kinase-3 beta (rhGSK-3β; aa 1-420; Accession # P49841).

Applications:
Immunocytochemistry

Recommended Dilution:
Immunocytochemistry: This antibody was used at a concentration of 25ug/mL with the appropriate secondary reagents to detect GSK-3β in fixed human MCF-7 and HeLa cells. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.