GM CSF Protein
Référence OPPA00487
Conditionnement : 5µg
Marque : Aviva Systems Biology
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GM CSF Protein (OPPA00487)
| Datasheets/Manuals | Printable datasheet for OPPA00487 |
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| Predicted Species Reactivity | Mouse |
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| Product Format | GM-CSF Mouse was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Host | E. Coli |
| Additional Information | Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| :: | Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5x106 IU/mg. |
| :: | Protein Content: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard. Protein Content: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard. |
| :: | Product Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Product Description: Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.GM-CSF Mouse is purified by proprietary chromatographic techniques. |
| Reconstitution and Storage | Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GM-CSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Met - Ala - Pro - Thr - Arg. |
| Gene Symbol | mGM CSF |
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| Alias Symbols | CSF, Csfg, GMCS, Csfgm, GMCSF, Gm-CS, MGI-I, Gm-CSf, MGI-IGM |
| NCBI Gene Id | 12981 |
| Protein Name | Granulocyte-macrophage colony-stimulating factor |
| Description of Target | Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor |
| Uniprot ID | P01587 |
| Protein Accession # | NP_034099.2 |
| Protein Size (# AA) | Recombinant |