Goat Anti-Mouse IgG, Human ads-AP
Référence MBS674383-5x1mL
Conditionnement : 5x1mL
Marque : MyBiosource
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Goat Anti-Mouse IgG (Gamma Chain Specific) Secondary Antibody – Goat Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674383MBS674383 is a secondary antibody targeting Goat Anti-Mouse IgG (gamma chain specific). Also known as Goat Anti-Mouse IgG, Human ads-AP. Reactive in Mouse. Host: Goat. More details
Product Overview
Product Name
Goat Anti-Mouse IgG (gamma chain specific), Secondary Antibody
Full Product Name
Goat Anti-Mouse IgG, Human ads-AP
Product Synonym Names
Goat Anti-Mouse IgG, Human ads- Alkaline Phosphatase-Labeled; Goat Anti-Mouse IgG (gamma chain specific)
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Mouse
Form/Format
Alkaline Phosphatase (AP) Conjugate
Purity/Purification
Affinity chromatography on pooled mouse IgG covalently linked to agarose.
Specificity
Reacts with mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Preparation and Storage
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The beta -galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS/NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The beta -galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS/NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Source
Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.
Cross Absorption
Mouse IgM and IgA, pooled human sera and purified human paraproteins.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Product Categories/Family for Goat Anti-Mouse IgG (gamma chain specific) secondary antibody
Applications Tested/Suitable for Goat Anti-Mouse IgG (gamma chain specific) secondary antibody
ELISA; Immunoblot; Immunostaining
Application Notes for Goat Anti-Mouse IgG (gamma chain specific) secondary antibody
Research Application(s): Indirect immunostaining in conjunction with mouse IgE monoclonal antibodies
ELISA Suggested Dilution: 1:2,000-1:4,000
ELISA Suggested Dilution: 1:2,000-1:4,000
Other Notes
Small volumes of Goat Anti-Mouse IgG (gamma chain specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.