Orb1146797-96 | Goat AST(Aspartate Aminotransferase) ELISA Kit Biovalley

Goat AST(Aspartate Aminotransferase) ELISA Kit

Catalog Number	orb1146797
Category	Assays and Kits
Description	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase(AST). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aspartate Aminotransferase(AST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase(AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Aminotransferase(AST) in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity	Goat
Range	0.16-10U/L
Sensitivity	0.055U/L
Protein Length	3.5h
Alternative names	cCAT, AAT, ASAT, SGOT, GOT1, Cysteine transaminase
Note	For research use only
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Expiration Date	Please enquire.