Growth Associated Protein 43 (GAP-43) (APC)

GAP-43 or growth associated protein 43 is a nervous system-specific phosphoprotein and a major component of motile growth cones which form the leading edge of the elongating axon. The membrane skeleton of the growth cone is responsible for transducing extracellular guidance cues into morphological alterations mediated by the polymerization/de-polymerization of actin resulting in directional changes in axon growth. GAP-43 is closely associated with the membrane skeleton and has been implicated in regulating interactions between cytoskeletal components and the plasma membrane. Interestingly, axonal transport of GAP-43 occurs only in neurons that are extending axons during development or regeneration after injury; however, this transport does not occur in axons that do not normally regenerate such as those in the mammalian central nervous system. These results suggest that the in vivo function of GAP-43 may be closely linked to axon growth and regeneration.

Applications:
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

WB Positive Controls:
Cell lysates derived from mouse and rat brain.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.