Growth Hormone Releasing Hormone (GHRH) Recombinant, Mouse

Recombinant protein corresponding to Leu20--Met97 of mouse GHRH with a N-terminal His-tag expressed in E. coli. Swiss/UniProt Accession: P16043

Amino Acid Sequence:
MGHHHHHHSGSEF-L PPSPPFRMQR HVDAIFTTNY RKLLSQLYAR KVIQDIMNKQ GERIQEQRAR LSRQEDSMWT EDKQMTLESI LQGFPRMKPS ADA

Molecular Weight:
11.5kD

Predicted Molecular Weight:
14.8kD

Accurate Molecular Mass:
16kD as determined by SDS-PAGE reducing conditions

Subcellular Location:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 10mM PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.