GST alpha Assay Kit
Référence MBS480433-5x1
Conditionnement : 5x1Kit
Marque : MyBiosource
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GST Alpha Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS480433Introduction: Glutathione S-Transferase (GST) has multiple isoforms. This assay is specific for Glutathione STransferase Alpha (GSTA) and is not known to cross react with the mu, pi, or theta variants. GSTA is a common biomarker for hepatocellular damage. 1… More details
Product Overview
Online Datasheet in PDFProduct Name
GST alpha, Assay Kit
Full Product Name
GST alpha Assay Kit
Product Gene Name
GST alpha assay kit
Product Synonym Names
Microplate Assay for GSTA
Preparation and Storage
1. Store the components of this kit at the temperatures specified on the labels.
2. Unopened reagents are stable until the indicated kit expiration date.
2. Unopened reagents are stable until the indicated kit expiration date.
Procedural Notes
1. Reagents can be used immediately upon removal from refrigeration.
2. To minimize errors in absorbance measurements due to handling, wipe the exterior bottom of the microplate wells with a lint-free paper towel prior to inserting into the plate reader.
3. Do not save excess diluted reagents or standards for future use.
2. To minimize errors in absorbance measurements due to handling, wipe the exterior bottom of the microplate wells with a lint-free paper towel prior to inserting into the plate reader.
3. Do not save excess diluted reagents or standards for future use.
Sample Collection and Preparation
Samples should be stored at -80 degree C and thawed just prior to use. Avoid repeated freeze/thaw cycles for best results. This assay was developed and validated with human serum samples, however it does crossreact with rat.
Reagent Preparation
1. Wash Buffer: Dilute the wash buffer 1:5 with dH2O. For example, if all the Wash Buffer will be used dilute the 40 mL with 160 mL of dH2O, giving a total final volume of 200 mL.
2. Antibody-HRP: Immediately prior to use, dilute the needed amount 1:25 with Assay Buffer. For example, if all will be used, dilute the complete 440 muL with 10.66 mL of Assay Buffer, giving a total final volume of 11 mL.
3. Standard: Perform a 1:100 dilution using Assay Buffer, giving a final concentration of 20 ng/mL. For example, add the 30 muL of Standard to 2.970 mL of Assay Buffer giving a total final volume of 3.0 mL.
2. Antibody-HRP: Immediately prior to use, dilute the needed amount 1:25 with Assay Buffer. For example, if all will be used, dilute the complete 440 muL with 10.66 mL of Assay Buffer, giving a total final volume of 11 mL.
3. Standard: Perform a 1:100 dilution using Assay Buffer, giving a final concentration of 20 ng/mL. For example, add the 30 muL of Standard to 2.970 mL of Assay Buffer giving a total final volume of 3.0 mL.
Sample Preparation
It is recommended to do multiple sample dilutions to ensure that the concentration falls within the accepted range for the assay. Samples are to be diluted in Assay Buffer. Dilution factors of 1:2 and 1:4 are normally sufficient.
Standard Curve Preparation
Set up for the standard curve by labeling dilution tubes and dispensing the indicated volumes of Assay Standard
GST
Concentration
(ng/mL)
Assay
Buffer
(L)
Volume of
20 ng/mL Standard
(L)
Final Volume
(L)
S6 20 - 1000 1000
S5 10 500 500 1000
S4 5.0 750 250 1000
S3 2.5 875 125 1000
S2 1.0 950 50 1000
S1 0.5 975 25 1000
B0 0.0 1000 - 1000 Buffer and diluted Standard according to Table 1 below.
GST
Concentration
(ng/mL)
Assay
Buffer
(L)
Volume of
20 ng/mL Standard
(L)
Final Volume
(L)
S6 20 - 1000 1000
S5 10 500 500 1000
S4 5.0 750 250 1000
S3 2.5 875 125 1000
S2 1.0 950 50 1000
S1 0.5 975 25 1000
B0 0.0 1000 - 1000 Buffer and diluted Standard according to Table 1 below.
Product References and Citations for GST alpha assay kit
1. Vaubourdolle, M, et al., Clinical Chemistry 41: 1716-1719, 1995
2. Awasthi, Y. C, et al., Free Radic. Biol. Med. 37: 607-619, 2004
3. Yang, Y., et al. Toxicol. Appl. Pharmacol. 182: 105-115, 2002
2. Awasthi, Y. C, et al., Free Radic. Biol. Med. 37: 607-619, 2004
3. Yang, Y., et al. Toxicol. Appl. Pharmacol. 182: 105-115, 2002
Other Notes
Small volumes of GST alpha assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GST alpha purchase
MBS480433 is an assay kit targeting GST alpha.