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> Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit

Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit

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Référence EK12252-48T

Conditionnement : 48T

Marque : Signalway Antibody

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Product Detail

Product NameHamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit

ApplicationsELISA

Species ReactivityHamster (Mesocricetus; Cricetulus)

Other NamesP76; LAMA-like protein 2|PLB homolog 2|lamina ancestor homolog 2|mannose-6-phosphate protein associated protein p76|phospholipase B-like 2 32 kDa form|phospholipase B-like 2 45 kDa form

StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

Application Details
Detect Range:Request Information
Sensitivity:Request Information
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLBD2 in samples. An antibody specific for PLBD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLBD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLBD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLBD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Most soluble lysosomal proteins carry Man6P (mannose 6-phosphate), a specific carbohydrate marker that enables their binding to cellular MPRs (Man6P receptors) and their subsequent targeting towards the lysosome.This characteristic was exploited to identify novel soluble lysosomal proteins by proteomic analysis of Man6P proteins purified from a human cell line. The presence of Man6P sugars was confirmed by an MPR overlay experiment, which showed the direct and Man6P-dependent interaction between p76 and the MPR. The presence of six N-glycosylation sites was validated by progressive peptide-N-glycosidase F deglycosylation. Experiments using N- and C-termini directed anti-p76 antibodies provided insights into p76 maturation.

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