Heat Shock Protein 20, Recombinant, Human (HSP20)

Dimers and Aggregates:|≤ 1% as determined by silver-stained SDS-PAGE gel analysis.||Immunological Activity:|Immunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSP-20.||Endotoxin:|≤ 0.1ng/ug (IEU/ug) of HSP-20.||Protein Content:|Protein quantitation was carried out by two independent methods: |1. UV spectroscopy at 280nm. |2. Analysis by RP-HPLC, using a calibrated solution of Human Heat Shock Protein as a Reference Standard.||Reconstitution:|Reconstitute the lyophilized HSP-20 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.||Storage and Stability:|Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.