HeLa/GFP Cell Line
Référence MBS168642-5x1
Conditionnement : 5x1Vial
Marque : MyBiosource
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Téléphone : +1 850 650 7790
HeLa/GFP Cell Line
HeLa/GFP Cell Line. Left: GFP Fluorescence; Right: Phase Contrast
HeLa/GFP Cell Line
For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS168642HeLa cells are the most widely used cancer cell lines in the world. These cells were taken from a lady called Henrietta Lacks from her cancerous cervical tumor in 1951 which today is known as the HeLa cells. These were the very first cell lines to survive… More details
Product Overview
Product Name
HeLa/GFP, Cell Line
Full Product Name
HeLa/GFP Cell Line
Product Synonym Names
HeLa/GFP Cell Line
Concentration
1 mL, 1 x 10^6 cells/mL in 70% DMEM, 20% FBS, 10% DMSO (lot specific)
Preparation and Storage
Liquid nitrogen.
Ships on dry ice. Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80°C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Ships on dry ice. Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80°C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Methods
Establishing HeLa/GFP Cultures from Frozen Cells
1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1-2 minutes by gentle agitation in a 37°C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37°C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1-2 minutes by gentle agitation in a 37°C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37°C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
Medium
1. Culture Medium: D-MEM (high glucose), 10% fetal bovine serum (FBS), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine, 1% Pen-Strep, (optional) 10 ug/mL Blasticidin.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
Quality Control
This cryovial contains at least 1.0 x 106 HeLa/GFP cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The HeLa/GFP cells are tested free of microbial contamination.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Product Categories/Family for HeLa/GFP cell line
Product References and Citations for HeLa/GFP cell line
Weerakkody, D. et al. (2013). Family of pH (low) Insertion Peptides for Tumor Targeting. PNAS. 110:5834-5839.
Other Notes
Small volumes of HeLa/GFP cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HeLa/GFP purchase
MBS168642 is a cell line targeting HeLa/GFP.