Heparan Sulfate, Sodium Salt, Bovine [57459-72-0]

Heparan sulfate, bovine, is specially prepared from bovine kidney, purified by a combination of chromatographic and chondroitinase ABC treatment. Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4)ß-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS-(1->4)-ß-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin.

Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4)alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc(1->4)-ß-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.

Synonyms:
Bovine Kidney; Heparitin Sulfate; Heparin Monosulfate; N–Acetylheparan Sulfate

CAS No:
57459-72-0

Source:
Bovine kidney

Purity:
Single band (TLC)

Form:
White to off-white powder

Total Nitrogen:
2.6-3.6%

Total Sulfur:
5-7%

Solubility (Turbidity,1mg/ml in water):
Clear to very slightly hazy

IR Spectrum:
Conforms to structure