Hepatitis B Core Antigen, IgM (HBV Core, HBcAg), ELISA Kit, BioAssay™

ELISA Kit for IgM Antibody to Hepatitis B Core Antigen is an in vitro enzyme immunoassay for the detection of Anti-HBc-IgM in human serum or plasma.

Principle:
The purified Anti-mu-chain is coated on the solid phase of multi-wells. Serum sample, HBcAg and Horseradish peroxidase labeled with Anti-HBc (conjugated) are added to coated wells. After incubation, if Anti-HBc-IgM is present in the sample, a complex of Anti-mu-chain-Anti-HBc-IgM-HBcAg-Anti-HBc labeled with HRP will form. Wash wells to remove other unbounded serum components, incubate with substrates (TMB) to form a colored product, and measure the absorbance at 450nm to indicate the presence or absence of Anti-HBc-IgM in the sample. The test is special, sensitive, reproducible and easy to operate.

Kit Components:
1. Anti-mu-chain Coated Microwell Plate
2. Enzyme Conjugant
3. Positive Control Serum
4. Negative Control Serum
5. Wash Buffer (1:20 dilution prior to use)
6. Substrate A
7. Substrate B
8. Stop Solution
9. HBcAg

Storage And Stability:
Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.