Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Hepatitis B Virus Capsid Protein (C) Human IgG ELISA Kit Standard Curve
Hepatitis B Virus Capsid Protein (C) Human IgG ELISA Kit
The Hepatitis B Virus Capsid Protein (C) Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG antibody against pathogen antigen(s) in human serum or plasma.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Hepatitis B Virus (HBV) |
| Detection Range | 4.096 Units/mL - 1000 Units/mL |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 1500-fold |
| Gene Symbols | IgG |
| Protein Name / Synonyms | IgG; Hepatitis B Virus Capsid Protein (C) |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Hepatitis |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -20°C |
Introduction
The Hepatitis B Virus Capsid Protein (C) Human IgG ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with a pathogen antigen, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgG antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control consists of an anti-Capsid antibody. A biotinylated anti-Positive Control antibody is added to the Positive Control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.
Kit Components
| Component | Size / Description |
|---|---|
| C Coated 96-Well Microplate | 96 wells coated with C |
| Wash Buffer | 25 mL |
| Positive Control | 1 vial |
| Biotinylated Anti-Human IgG | 1 vial |
| HRP-Streptavidin | 1 vial |
| TMB One-Step Substrate Reagent | 12 mL |
| Stop Solution | 8 mL |
| Assay Diluent | 15 mL |
| Sample Diluent | 25 mL |
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 100 µL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Biotinylated Anti-Human IgG Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.