Histone H2Av antibody (mAb)(Clone 10E9.D1)
Référence MBS389035-5x0,1mg
Conditionnement : 5x0.1mg
Marque : MyBiosource
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Histone H2Av antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from Drosophila cells and 5 ug of H2Av antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
Histone H2Av antibody (mAb) tested by Western blot. Detection of Histone H2Av by Western blot. The analysis was performed using Schneider’s Drosophila L2 nuclear extract (20ug)and the Histone H2Av antibody at a dilution of 1ug/ml.
Histone H2av (H2Av) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS389035Short Description: Our Histone H2Av antibody (mAb)(Clone 10E9.D1) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Drosophila samples.Background: The nucleosome is the… More details
Product Overview
Online Datasheet in PDFProduct Name
Histone H2Av, Monoclonal Antibody
Full Product Name
Histone H2Av antibody (mAb)(Clone 10E9.D1)
Product Gene Name
anti-H2Av antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10E9.D1
Host
Mouse
Species Reactivity
Drosophila
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Protein A Chromatography
Concentration
1ug/ul (lot specific)
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Molecular Weight
15kDa
Immunogen
This antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster Histone variant H2Av.
Product Categories/Family for anti-H2Av antibody
Primary Antibodies
Applications Tested/Suitable for anti-H2Av antibody
Chromatin Immunoprecipitation (ChIP), Western Blot (WB)
Application Notes for anti-H2Av antibody
Applications Validated:
ChIP: 5ug per ChIP
WB: 0.5-2ug/ml dilution
ChIP: 5ug per ChIP
WB: 0.5-2ug/ml dilution
Chromatin Immunoprecipitation (ChIP) of anti-H2Av antibody
Histone H2Av antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from Drosophila cells and 5 ug of H2Av antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
Other Notes
Small volumes of anti-H2Av antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for H2Av purchase
MBS389035 is an antibody targeting H2Av. Also known as Histone H2Av antibody (mAb)(Clone 10E9.D1). Reactive in Drosophila. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for H2Av. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI Related Accession #
Protein Family
Histone
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.