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Histones (FITC)

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Référence H5300-10-FITC-100ul

Conditionnement : 100ul

Marque : US Biological

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H5300-10-FITC Histones (FITC)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P10412
Isotype
IgG2a,k
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Bo Hm Hu
Accession #
NM_005321.2
Shipping Temp
Blue Ice
Storage Temp
-20°C

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H1 is located in the nuclear and is necessary for the condensation of nucleosome chains into higher order structures. Histone H1 produces a speckled staining pattern in the nucleus of normal and malignant cells.

Applications:
Suitable for use in FLISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Positive Control:
Jurkat cell lysate

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: 2Q2205 (F152.C25.WJJ)|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Histones purified from calf thymus. MW: 15-33kD.|Specificity: Recognizes human Histones, H1 and core proteins H2a, H2b, H3, H4. Species Crossreactivity: bovine and hamster||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Histones purified from calf thymus. MW: 15-33kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Histones, H1 and core proteins H2a, H2b, H3, H4. Species Crossreactivity: bovine and hamster
References
1. Federation Proceedings (1984). 43:2063. 2. Mizoguchi, E., et al., Antineutrophil Cytoplamic Antibodies in T-Cell Receptor •-Deficient Mice with Chronic Colitis. Gastroenterology (1997) 113:1828–1835. 3. Feng, H., et al., CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans. Nature Cell Biology (1999) 1:486- 492. 4. Brimmell, M., et al., High level expression of differentially localized BAG-1 isoforms in some oestrogen receptor-positive human breast cancers. British Journal of Cancer (1999) 81:1042–1051.