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> Histones, pan (APC)

Histones, pan (APC)

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Référence H5305-APC-100ul

Conditionnement : 100ul

Marque : US Biological

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H5305-APCHistones, pan (APC)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
IsotypeIgG1
GradeAffinity Purified
ApplicationsIC IHC WB
CrossreactivityBo Hm Hu Op Rt Xe
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: Detects a band at ~20kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2Q2283|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Purified nuclear fractions of HeLa cells.|Specificity: Recognizes human histones H1, H2A, H2B, H3 and H4 with comparable sensitivity. Binds specifically to an antigen determinant, which is present on all five histone proteins. Species Crossreactivity: bovine, rat, Xenopus, opossum and hamster. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified nuclear fractions of HeLa cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human histones H1, H2A, H2B, H3 and H4 with comparable sensitivity. Binds specifically to an antigen determinant, which is present on all five histone proteins. Species Crossreactivity: bovine, rat, Xenopus, opossum and hamster.
References
1. Kutney, S., et al. (2004). J. Biol. Chem. 279(29): 30850-30855.