Histones, pan (Biotin)

There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: Detects a band at ~20kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.