HLA-DRB1 Antibody - Biotin Conjugated
Référence OACA02248
Conditionnement : 100ug
Marque : Aviva Systems Biology
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HLA-DRB1 Antibody - Biotin Conjugated (OACA02248)
| Datasheets/Manuals | Printable datasheet for HLA-DRB1 Antibody - Biotin Conjugated (OACA02248) (OACA02248) |
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| Predicted Species Reactivity | Human |
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| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Biotin |
| Application | ELISA, IHC |
| Reconstitution and Storage | -20°C or -80°C |
| Immunogen | Recombinant Human HLA class II histocompatibility antigen, DRB1-3 chain protein (31-266AA) |
| Purification | Protein G Purified |
| Purity | Caprylic Acid Ammonium Sulfate Precipitation purified |
| Storage Buffer | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reference | [1] Mutations and selection in the generation of class II histocompatibility antigen polymorphism.'Peterson P.A., Gustafsson K., Wiman K.G., Emmoth E., Larhammar D., Boehme J., Hyldig-Nielsen J.J., Ronne H., Rask L.EMBO J. 3:1655-1660(1984) [2] Molecular analysis of human class II transplantation antigens and their genes.'Larhammar D., Andersson G., Andersson M., Bill P., Boehme J., Claesson L., Denaro M., Emmoth E., Gustafsson K., Hammarling U., Heldin E., Hyldig-Nielsen J.-J., Lind P., Schenning L., Servenius B., Widmark E., Rask L., Peterson P.A.Hum. Immunol. 8:95-103(1983) [3] Invariant chain structure and MHC class II function.'Cresswell P.Cell 84:505-507(1996) |
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| Gene Symbol | HLA-DRB1 |
| Gene Full Name | major histocompatibility complex, class II, DR beta 1 |
| Alias Symbols | Clone P2-beta-3;DRB1;HLA class II histocompatibility antigen, DR-1 beta chain;HLA-DR1B;HLA-DRB;human leucocyte antigen DRB1;Human leukocyte antigen DRB1;lymphocyte antigen DRB1;major histocompatibility complex, class II, DR beta 1 precursor;MHC class II antigen DRB1*3;MHC class II HLA-DR beta 1 chain;SS1. |
| NCBI Gene Id | 3123 |
| Protein Name | HLA class II histocompatibility antigen, DRB1 beta chain|HLA class II histocompatibility antigen, DRB1-3 chain |
| Description of Target | A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA-DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed:29884618, PubMed:22327072, PubMed:27591323, PubMed:8642306, PubMed:15265931, PubMed:31495665, PubMed:16148104). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed:8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed:31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed:17182262, PubMed:23783831). The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed:25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed:8145819).|Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. |
| Uniprot ID | P01912 |
