HSP60 (Heat Shock Protein 60, 60kD chaperonin, 60kD heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)) (PE)
Référence 168797-AF-PE-100ul
Conditionnement : 100ul
Marque : US Biological
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
168797-AF-PE HSP60 (Heat Shock Protein 60, 60kD chaperonin, 60kD heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)) (PE)
Clone Type
MonoclonalHost
mouseSource
humanSwiss Prot
P10809 (Human); P63038 (Mouse); P63039 (Rat)Isotype
IgG1,kGrade
PurifiedApplications
FC IF IHC IP WBCrossreactivity
Bo Ca Ch Dr Hm Hu Mk Mo Po Rb Rt Sh XeShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeRecognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp’s), more appropriately called as stress response proteins (srp’s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 0.5-1ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 0.5-1ug/500ug protein lysate
Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control:
HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.
Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.