RayBio® Human Adiponectin (ACRP30) ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Human Adiponectin ELISA Kit
Human Adiponectin ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 25 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 25pg/ml - 18,000pg/ml |
| Specificity | Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 30,000 fold |
| Accession Number | Q15848 |
| Gene Id | 9370 |
| Gene Symbols | ADIPOQ, ACRP30, ACDC, APM1, GBP28 |
| Protein Name / Synonyms | Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipose most abundant gene transcript 1 protein) (apM-1) (Gelatin-binding protein) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Adiponectin is a 244-amino acid protein secreted mainly by adipose tissue, but expression is also seen in osteoblasts, liver parenchyma cells, myocytes, epithelial cells, and placental tissue. The biological actions of adiponectin are directed through the receptors AdipoR1, AdipoR2 and T-cadherin. Serum levels of adiponectin decrease with obesity and are positively linked with insulin sensitivity. Low levels of this anti-inflammatory adipokine can produce a chronic state of low-grade inflammation which can lead to insulin resistance, diabetes, hypertension, and atherosclerosis.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Adiponectin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Adiponectin (ACRP30 / ADIPOQ), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
The utility of early gestational OGTT and biomarkers for the development of gestational diabetes mellitus: an international prospective multicentre cohort study
- Species:
- Human
- Sample Type:
- Serum (maternal serum)
- Year:
- 2025
- Journal Name:
- Diabetologia
- PubMed ID:
- 40817933
Associations of adiponectin, leptin, and the adiponectin-to-leptin ratio with sarcopenia in older adults with cardiovascular-kidney-metabolic syndrome
- Species:
- Human
- Sample Type:
- Plasma (older adults with CKM syndrome)
- Year:
- 2026
- Journal Name:
- The journal of nutrition, health & aging
- PubMed ID:
- 42385583
Effects of 12-Week Resistance Training on Metabolic and Immune Responses in Normal-Weight and Older Women with Obesity
- Species:
- Human
- Sample Type:
- Serum (older women, normal-weight and obese)
- Year:
- 2026
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 42737494
Periodontitis in Patients With Severe Obesity: From the Oral and Gut Microbiota Dysregulation to the Visceral Adipose Tissue Inflammatory and Metabolic Disorders
- Species:
- Human
- Sample Type:
- Plasma; Tissue Lysate / Homogenate (adipose tissue protein lysates); Saliva
- Year:
- 2026
- Journal Name:
- FASEB Journal
- PubMed ID:
- 42037322
Evaluating Pro- and Anti-inflammatory Biomarkers for Predicting Type 2 Diabetes Mellitus in the Geriatric Population and Correlation With Clinical and Biochemical Parameters
- Species:
- Human
- Sample Type:
- Serum (geriatric subjects, fasting)
- Year:
- 2025
- Journal Name:
- Curēus
- PubMed ID:
- 39991369
Bariatric Surgery Induces Alterations in the Immune Profile of Peripheral Blood T Cells
- Species:
- Human
- Sample Type:
- Plasma (bariatric surgery patients)
- Year:
- 2024
- Journal Name:
- Biomolecules
- PubMed ID:
- 38397455
Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals
- Species:
- Human
- Sample Type:
- Serum (obese individuals)
- Year:
- 2024
- Journal Name:
- Diabetology & Metabolic Syndrome
- PubMed ID:
- 39468643
Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence
- Species:
- Human
- Sample Type:
- Serum (bone marrow serum); Bone Marrow
- Year:
- 2021
- Journal Name:
- Nature Communications
- PubMed ID:
- 34535653
The influence of xeno-free culture conditions on the angiogenic and adipogenic differentiation properties of adipose tissue-derived stem cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (adipogenic-differentiated ADSCs, day 21)
- Year:
- 2024
- Journal Name:
- Regenerative Therapy
- PubMed ID:
- 39822342
Blenderized watermelon consumption decreases body mass index, body mass index percentile, body fat and HbA1c in children with overweight or obesity
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Pediatric Obesity