Semi-quantitative, sandwich-based, membrane cytokine array to detect 20 human angiogenesis protein biomarkers.
Human Angiogenesis Array C1
Human Angiogenesis Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Angiogenin
| EGF
| ENA-78 (CXCL5)
| bFGF
| GRO alpha/beta/gamma
|
| IFN-gamma
| IGF-1
| IL-6
| IL-8 (CXCL8)
| Leptin
|
| MCP-1 (CCL2)
| PDGF-BB
| PLGF
| RANTES (CCL5)
| TGF beta 1
|
| TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
| VEGF-A
| VEGF-D
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Human Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Three-dimensional culture conditioned bone marrow MSC secretome accelerates wound healing in a burn injury mouse model
- Species:
- Human
- Sample Type:
- Mesenchymal stem cell (MSC)
- Year:
- 2023
- Journal Name:
- Biochemical and Biophysical Research Communications
A New Osteogenic Membrane to Enhance Bone Healing: At the Crossroads between the Periosteum, the Induced Membrane, and the Diamond Concept
- Species:
- Pig
- Sample Type:
- periosteal mesenchymal stem cells (PMSCs)
- Year:
- 2023
- Journal Name:
- Bioengineering-Basel
Glioblastoma CD105+ cells define a SOX2− cancer stem cell-like subpopulation in the pre-invasive niche
- Species:
- Human
- Sample Type:
- Glioblastoma (GBM) cell
- Year:
- 2022
- Journal Name:
- Acta neuropathologica communications
Effect of Aspirin on G0/G1 Cell Cycle Arrest and microRNA Signatures in Pancreatic Adenocarcinoma Cells
- Species:
- Human
- Sample Type:
- PANC-1 cell, PK-8 cell
- Year:
- 2022
- Journal Name:
- Anticancer Research
miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (Gall bladder cancner cell line, NOZ treated with microRNAs)
- Year:
- 2018
- Journal Name:
- Cell Death and Disease
Inflammation and N-formyl peptide receptors mediate the angiogenic activity of human vitreous humour in proliferative diabetic retinopathy
- Species:
- Human
- Sample Type:
- Vitreous Fluid (Proliferative diabetic retinopathy)
- Year:
- 2017
- Journal Name:
- Diabetologia
- PubMed ID:
- 28083635
Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Bone Marrow fibroblasts and MKN28 gastric cancer cell lines)
- Year:
- 2016
- Journal Name:
- Oncogene
IKBB tumor suppressive role in nasopharyngeal carcinoma via NF-B-mediated signalling
- Species:
- Human
- Sample Type:
- Conditioned Media (nasopharyngeal carcinoma cell lines with or without knockdown to IkappaB, NFkB, etc.)
- Year:
- 2016
- Journal Name:
- International Journal of Cancer
- PubMed ID:
- 26227166
Galectin-9 suppresses the proliferation of gastric cancer cells in vitro
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Gastric Cancer cell lines treated with Galectin 9)
- Year:
- 2016
- Journal Name:
- Oncology Reports
- PubMed ID:
- 26717877
Apoptotic and anti-angiogenic effects of Salvia triloba extract in prostate cancer cell lines
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Prostate cancer cells lines PC-3, DU-145, and HUVEC, and MCF-10A cells)
- Year:
- 2016
- Journal Name:
- Tumor Biology
- PubMed ID:
- 26459311