RayBio® G-Series Human Angiogenesis Antibody Array 1000 Kit. A combination of Human Angiogenesis Antibody Array G1 & G2. Detects 43 Human Angiogenic Factors. Suitable for all liquid sample types.
Human Angiogenesis Array G1000
Human Angiogenesis Array G1000
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 43 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| Angiopoietin-1
| Angiopoietin-2
| Angiostatin
| bFGF
|
EGF
| ENA-78 (CXCL5)
| Endostatin
| GCSF
| GM-CSF
|
GRO alpha/beta/gamma
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
| IL-10
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-4
| IL-6
|
IL-8 (CXCL8)
| I-TAC (CXCL11)
| Leptin
| MCP-1 (CCL2)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| MMP-1
| MMP-9
| PDGF-BB
| PECAM-1 (CD31)
|
PLGF
| RANTES (CCL5)
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-2
|
TIMP-1
| TIMP-2
| TNF alpha
| uPAR
| VEGF-A
|
VEGFR2
| VEGFR3
| VEGF-D
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Angiogenesis Antibody Array G1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-ANG-G1-4.gal)
GAL (GAL-AAH-ANG-G1-8.gal)
GAL (GAL-AAH-ANG-G2-4.gal)
GAL (GAL-AAH-ANG-G2-8.gal)
Excel (AAH-ANG-1_Analysis_Tool.xls)
Excel (AAH-ANG-2_Analysis_Tool.xls)
citations
Analysis of vascular-associated factors and the prognosis of poorly differentiated hypopharyngeal carcinoma
- Species:
- Human
- Sample Type:
- Tissue Lysate
- Year:
- 2020
- Journal Name:
- Oncology Letters
Microvesicles of pregnant women receiving low molecular weight heparin improve trophoblast function
- Species:
- Human
- Sample Type:
- Conditioned Media (Microvesicle specimens)
- Year:
- 2016
- Journal Name:
- Thrombosis Research
- PubMed ID:
- 26639203
Angiogenin secretion from hepatoma cells activates hepatic stellate cells to amplify a self-sustained cycle promoting liver cancer
- Species:
- Human
- Sample Type:
- Conditioned Media (HepG2, Hep3B, and LX2 cells)
- Year:
- 2015
- Journal Name:
- Scientific reports
- PubMed ID:
- 25604905
Three-Dimensional Platelet-Rich Plasma Hydrogel Model to Study Early Tendon Healing
- Species:
- Human
- Sample Type:
- Conditioned Media (2d cultures of tendons and platelet rich plasma)
- Year:
- 2015
- Journal Name:
- Cells Tissues Organs
- PubMed ID:
- 26562323
Control of Angiogenesis by Galectins Involves the Release of Platelet-Derived Proangiogenic Factors
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Platelets, cultured media)
- Year:
- 2014
- Journal Name:
- Plos One
Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes
- Species:
- Human
- Sample Type:
- Conditioned Media (Colon adenocarcinoma LS174T cells co-culutred with peropheral blood lymphocytes )
- Year:
- 2012
- Journal Name:
- International Journal of Radiation Oncology • Biology • Physics
- PubMed ID:
- 22712702