Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
Sterile tubes and Eppendorf tubes with disposable tips
Absorbent paper and loading slot
Deionized or distilled water
Standard Curve
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml)
OD-1
OD-2
Average
0
0.094
0.099
0.096
1.562
0.169
0.177
0.172
3.125
0.229
0.241
0.234
6.25
0.361
0.379
0.368
12.5
0.613
0.645
0.626
25
0.99
1.04
1.01
50
1.589
1.67
1.621
100
2.092
2.199
2.135
Recovery
Add a certain amount of Anti-MPV IgM into the sample. Calculate the recovery by comparing the measured value with the expected amount of Anti-MPV IgM in the sample.
Sample Type
Recovery Range(%)
Average(%)
serum(n=10)
97-105
99
EDTA plasma(n=10)
95-104
98
Heparin plasma(n=10)
96-99
97
Linearity
Dilute the sample with a certain amount of Anti-MPV IgM at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type
1:2
1:4
1:8
serum(n=10)
93-105%
93-99%
91-100%
EDTA plasma(n=10)
89-102%
89-101%
90-99%
Heparin plasma(n=10)
93-102%
89-98%
91-100%
Precision(%)
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/ml)
3.18
11.3
47.28
3.1
11.72
48.59
Standard deviation
0.15
0.45
2.42
0.17
0.62
2.27
CV(%)
4.66
3.95
5.12
5.43
5.31
4.68
Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.