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> Human ApoB ELISA Kit

Human ApoB ELISA Kit

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Référence ELH-ApoB-2

Conditionnement : 2PlateKit

Marque : RayBiotech

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Téléphone : +1 850 650 7790

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Human ApoB ELISA KitHuman ApoB ELISA KitHuman ApoB ELISA KitHuman ApoB ELISA KitHuman ApoB ELISA KitHuman ApoB ELISA Kit5 Plate Kit Packaging (one box)

Human ApoB ELISA Kit

Human ApoB ELISA Kit

RayBio® Human ApoB ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
0.21 ng/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.21 ng/ml - 50 ng/ml
Specificity
This ELISA antibody pair detects Human ApoB. Other species not determined.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
20,000 fold
Accession Number
P04114
Gene Id
338
Gene Symbols
APOB
Protein Name / Synonyms
Apolipoprotein B-100 (Apo B-100) [Cleaved into: Apolipoprotein B-48 (Apo B-48)]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human ApoB ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified Apolipoprotein B (ApoB), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-ApoB.pdf)
SDS (ELH-ApoB.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • The COPI coatomer influences LDL receptor activity, hepatic lipid storage, and apoB secretion

    Species:
    Human
    Sample Type:
    Other / Unspecified (harvested media from Huh-7 cells)
    Year:
    2026
    Journal Name:
    Atherosclerosis
    PubMed ID:
    42282770

  • Hereditary angioedema plasma proteomics following specific plasma kallikrein inhibition with lanadelumab

    Species:
    Human
    Sample Type:
    Plasma (HAE-C1INH patients and healthy controls)
    Year:
    2024
    Journal Name:
    Frontiers in Immunology
    PubMed ID:
    40417315

  • Simple and Fast SEC-Based Protocol to Isolate Human Plasma-Derived Extracellular Vesicles for Transcriptional Research

    Species:
    Human
    Year:
    2020
    Journal Name:
    Molecular Therapy-Methods & Clinical Development
    PubMed ID:
    32913880

  • A comparative study on the lipidome of normal knee synovial fluid from humans and horses

    Species:
    Human
    Sample Type:
    Other Body Fluid (synovial fluid)
    Year:
    2021
    Journal Name:
    PLoS ONE
    PubMed ID:
    33861772

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