ELH-BNGF-2 | Human beta-NGF ELISA Kit Biovalley

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Human beta-NGF ELISA Kit Standard Curve

Human beta-NGF ELISA Kit

RayBio® Human beta-NGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Sensitivity	14 pg/ml Need more sensitivity? Check out the new NexaTag^® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	14 pg/ml - 5000 pg/ml
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)	2 fold
Accession Number	P01138
Gene Id	4803
Gene Symbols	NGF, NGFB
Protein Name / Synonyms	Beta-nerve growth factor (Beta-NGF)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Shipping Type	Blue ice
Storage	≤-20°C

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Target Background

Nerve growth factor β (BNGF) is a neurotrophin essential for the development and functional maintenance of neurons in the central (cholinergic) and peripheral nervous system. It is translated as a precursor pro-protein which undergoes cleavage to produce a biologically active fragment, which is then homodimerized and secreted. The main receptor for BNGF is tyrosine kinase receptor A (TRKA). Mutations in the BNGF gene are associated with loss of pain perception (hereditary, sensory and autonomic neuropathy, type 5 (HSAN5)), mental retardation and immune deficiency.

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

The Human β-NGF ELISA Kit was used to quantify NGF released from leukocyte- and platelet-rich fibrin (L-PRF) in exudate and conditioned media over time. Figure adapted from Lambrichts et al., International Journal of Molecular Sciences, 2023.

The Human β-NGF ELISA Kit was used to quantify β-NGF release kinetics from injectable microrods for localized delivery. Figure adapted from Rivera et al., Frontiers in Bioengineering and Biotechnology, 2023.

The Human β-NGF ELISA Kit (ELH-BNGF) was used to quantify NGF levels in serum and urine from rats exposed to Water Avoidance Stress (WAS). Figure adapted from Dias et al., Scientific Reports, 2019.

The Human β-NGF ELISA Kit (ELH-BNGF) was used to quantify NGF secretion in conditioned media from glioma-initiating cells following CDK4/6 inhibitor treatment. Figure adapted from Olmez et al., Cancer Research, 2018.

The Human β-NGF ELISA Kit (ELH-BNGF) quantified NGF protein secretion from human intervertebral disc cells after inflammatory stimulation. Figure adapted from Krock et al., Journal of Biological Chemistry, 2016.

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Additional Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human beta-NGF ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified beta-NGF, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-BNGF.pdf)

SDS (ELH-BNGF.pdf)

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

Plasma nerve growth factor level is associated with functional outcome after ischemic stroke
Year:
2026
Journal Name:
Clinica Chimica Acta
PubMed ID:
42456845
A BDNF-TrkB autocrine loop enhances senescent cell viability
Species:
Human
Sample Type:
WI-38 cell, BJ cell, IMR-90 cell
Year:
2022
Journal Name:
Nature Communications
Oral Peroxisome Proliferator–Activated Receptor-α Agonist Enhances Corneal Nerve Regeneration in Patients With Type 2 Diabetes
Species:
Human
Sample Type:
tear
Year:
2022
Journal Name:
Diabetes
Cytokine profile of cerebrospinal fluid in pediatric patients with metastatic medulloblastoma
Species:
Human
Sample Type:
Cerebrospinal Fluid
Year:
2024
Journal Name:
Heliyon
PubMed ID:
39524698
Encapsulation of β-NGF in injectable microrods for localized delivery accelerates endochondral fracture repair
Species:
Human
Sample Type:
TF-1 cell
Year:
2023
Journal Name:
Frontiers in bioengineering and biotechnology
The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons—Implications for Biomaterial Applicability
Species:
Human
Sample Type:
Leukocyte- and Platelet Rich Fibrin (L-PRF)
Year:
2023
Journal Name:
International Journal of Molecular Sciences
Characterizing the impact of 2D and 3D culture conditions on the therapeutic effects of human mesenchymal stem cell secretome on corneal wound healing in vitro and ex vivo
Year:
2019
Journal Name:
Acta Biomaterialia
PubMed ID:
31539656
Combined c-Met/Trk Inhibition Overcomes Resistance to CDK4/6 Inhibitors in Glioblastoma
Year:
2018
Journal Name:
Cancer Research
PubMed ID:
29844123
Therapeutic Effect of Human Mesenchymal Stem Cell-Conditioned Medium on Erectile Dysfunction
Species:
Human
Sample Type:
Cell Culture Supernatant (CM mesenchymal stem cell-conditioned medium)
Year:
2022
Journal Name:
The world journal of men's health
PubMed ID:
35021313
Lineage-Specific Commitment of Stem Cells with Organic and Graphene Oxide-Functionalized Nanofibers
Species:
Human
Sample Type:
Conditioned Media (Dental Pulp stem cells)
Year:
2018
Journal Name:
Advances in simulation (London, England)

Human beta-NGF ELISA Kit

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Human beta-NGF ELISA Kit