Human CD11a/LFA-1a Antibody : SPRD
Référence OASB00920
Conditionnement : 100tests
Marque : Aviva Systems Biology
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Human CD11a/LFA-1a Antibody : SPRD (OASB00920)
| Datasheets/Manuals | Printable datasheet for Human CD11a/LFA-1a Antibody : SPRD (OASB00920) |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | 38 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | Spectral Red® |
| Application | FC |
| Additional Information | Description: CD11a, also known as leukocyte function-associated antigen-1 (LFA-1alpha), represents the 180 kDa integrin alphaL subunit which combines with the CD18 beta2 integrin subunit to form the alphaL/beta2 integrin heterodimer. It is expressed on lymphocytes, granulocytes, monocytes, and macrophages and is upregulated on activated T cells. CD11a mediates adhesion of lymphocytes to vascular endothelium and is involved in costimulation. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Fibronectin purified monocytes |
| Concentration | Lot specific |
| Specificity | CD11a |
| Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Fluorescein conjugate Biotin conjugate R-PE, APC, and SPRD conjugates <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Studies in cell adhesion and cytotoxicity, Immunohistochemisty (frozen sections), Immunoprecipitation, Blocking studies |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates aresupplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin/azide- free antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS;aliquot and freeze upon receipt - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Gene Symbol | ITGAL |
|---|---|
| Gene Full Name | Integrin alpha-L |
| Alias Symbols | CD11A, LFA-1, LFA1A |
| NCBI Gene Id | 3683 |
| Protein Name | integrin alpha-L |
| Description of Target | ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. |
| Uniprot ID | P20701 |
| Protein Accession # | NP_001107852.1 |
| Nucleotide Accession # | NM_001114380.1 |
