Human CD20 Antibody : APC/Cy7
Référence OASB00945
Conditionnement : 100tests
Marque : Aviva Systems Biology
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Human CD20 Antibody : APC/Cy7 (OASB00945)
| Datasheets/Manuals | Printable datasheet for Human CD20 Antibody : APC/Cy7 (OASB00945) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | B-Ly1 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | APC-Cyanine 7 |
| Application | FC |
| Additional Information | Description: CD20 is a 33-37 kDa unglycosylated phosphoprotein. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. It is expressed on all stages of B cell differentiation except plasma cells. The CD20 molecule is involved in regulation of B cell differentiation presumably via its reported function as a Ca++ channel subunit. |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | Lot specific |
| Specificity | CD20 |
| Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate R-phycoerythrin-Cyanine 7 conjugate APC/ CY7 <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunoprecipitation, In vitro activation of B cells |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE), and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), and allophycocyanin- Cyanine7 (APC- CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD20 Section, Academic Press, New York, p. 181 3. Tedder. T.F., and P. Engel. 1994. Immunol. Today 15:450 4. Tedder, T.F., M. Streuli, S.F. Schlossman, and H. Saito. 1988. Proc. Natl. Acad. Sci. USA 85:208. |
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| Gene Symbol | MS4A1 |
| Gene Full Name | B-lymphocyte antigen CD20 |
| Alias Symbols | B1, S7, Bp35, CD20, FMC7, CVID5, MS4A2, LEU-16 |
| NCBI Gene Id | 931; 494216 |
| Protein Name | B-lymphocyte antigen CD20 |
| Description of Target | This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. |
| Uniprot ID | P11836, Q5R1M8 |
| Protein Accession # | NP_068769.2 |
| Nucleotide Accession # | NM_021950.3 |
