Human CD4 Antibody : LE/AF
Référence OASB01002
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Human CD4 Antibody : LE/AF (OASB01002)
| Datasheets/Manuals | Printable datasheet for Human CD4 Antibody : LE/AF (OASB01002) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | RFT4 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, ICC, WB |
| Additional Information | Description: CD4 is a 55 kDa type I transmembrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is expressed predominantly on the "helper/inducer" subpopulation of mature T lymphocytes and on monocytes and macrophages. Domains 1 and 2 bind to MHC Class II antigens, while domains 3 and 4 may be involved in cis interactions with the CD3/TCR complex. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC class II antigens by T cells. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | Thymocytes and E-rosetted lymphocytes |
| Concentration | 0.5 mg/mL |
| Specificity | CD4 |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate R-phycoerythrin conjugate-Texas Red® Allophycocyanin conjugate Spectral Red conjugate Cyanine 5 conjugate R-PE/CY 5.5 and R-PE/CY7conjugate Alexa Fluor 647 (AF647 conjugate Allophycocyanin -Cyanine 7 conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation |
| Other Applications Data | Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), Alexa Fluor 488 (AF488), Alexa Fluor 647 (AF647), and Cyanine 5 (CY 5) conjugates are supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and Allophycocyanin (APC) conjugates are supplied as100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), R- phycoerythrin- Cyanine 7 (R- PE- CY 7) and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. |
| Reference | 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, OxfordUniversity Press, Oxford 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD4 Section, Academic Press, New York, p. 141 3. Parne, J.R. 1984. Adv. Immunol. 44:265 4. Littman, D.R., ed. 1996. The CD4 Molecule. In: Curr. Top. Microbiol. Immunol. 205 5. Sakihama, T., A. Smolyar, and E.L. Reinherz. 1995. Immunol. Today 16:581 6. Vignali, D.A., R.T. Carson, B. Chang, R.S. Mittler, and J.L. Strominger. 1996. J. Exp. Med. 183:2097. |
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| Gene Symbol | CD4 |
| Gene Full Name | T-cell surface glycoprotein CD4 |
| Alias Symbols | IMD79, OKT4D, CD4mut |
| NCBI Gene Id | 920 |
| Protein Name | T-cell surface glycoprotein CD4 |
| Description of Target | This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
| Uniprot ID | P01730 |
| Protein Accession # | NP_000607.1 |
| Nucleotide Accession # | NM_000616.4 |
