Human CD71 Antibody : LE/AF
Référence OASB01135
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Human CD71 Antibody : LE/AF (OASB01135)
| Datasheets/Manuals | Printable datasheet for Human CD71 Antibody : LE/AF (OASB01135) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | RVS-10 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, ICC |
| Additional Information | Description: CD71 is a 95 kDa type II heterodimeric transmembrane glycoprotein that is the receptor for transferrin. CD71 expression is very low on resting leukocytes but is upregulated upon activation. CD71 plays a critical role in cell proliferation by controlling the supply of iron which is essential for many metabolic pathways through the binding and endocytosis of transferrin. |
| Reconstitution and Storage | Store at -20C |
| Concentration | 0.5 mg/mL |
| Specificity | CD71 |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified (UNLB) antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate APC conjugate Spectral Red conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and Allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
| Reference | 1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD71 Section, Academic Press, New York, p. 3202. Tetsa, U., E. Pelosi, and C. Peschle 1993. Crit. Rev. Oncog. 4:241 3. Trowbridge, I.S., J.F. Collawn, and C.R. Hopkins. 1993. Annu. Rev. Cell Biol. 9:129 4. Hentze, M.W., and L.C. Kuhn. 1996. Proc. Natl. Acad. Sci. USA 93:8175. |
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| Gene Symbol | TFRC |
| Gene Full Name | Transferrin receptor protein 1 |
| Alias Symbols | T9, TR, TFR, p90, CD71, TFR1, TRFR, IMD46 |
| NCBI Gene Id | 7037 |
| Protein Name | transferrin receptor protein 1 |
| Description of Target | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. |
| Uniprot ID | P02786 |
| Protein Accession # | NP_001121620.1 |
| Nucleotide Accession # | NM_001128148.2 |
