Human CD8 Antibody : LE/AF
Référence OASB01025
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Human CD8 Antibody : LE/AF (OASB01025)
| Datasheets/Manuals | Printable datasheet for Human CD8 Antibody : LE/AF (OASB01025) |
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| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | RFT8 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IHC-P, ICC |
| Additional Information | Description: CD8 is a 33 kDa transmembrane glycoprotein expressed as either a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer. It is expressed on the "cytotoxic/suppressor" subpopulation of peripheral T cells. CD8 functions primarily as a coreceptor with MHC class I-restricted TCR's in antigen recognition. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | Thymocytes and E-rosetted lymphocytes |
| Concentration | 0.5 mg/mL |
| Specificity | CD8 |
| Characterization | To insure acceptable performance, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate R-phycoerythrin conjugate-Texas Red® Allophycocyanin conjugate Spectral Red conjugate Cyanine 5 conjugate PE/CY5.5 and PE/CY7 conjugates APC/CY5.5 and APC/CY7conjugate s AF488 and AF647 conjugates <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
| Application Info | Flow cytometry, Immunohistochemistry (frozen sections) |
| Other Applications Data | Since applications vary, investigators are advised to determine theoptimum working dilution of the product that is appropriate for a specificuse. |
| Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), Cyanine 5 (CY 5), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647)conjugates are supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as100 tests in 1.0 mLof PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7(R- PE- CY 7), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), Allophycocyanin- Cyanine 5.5 (APCCY 5.5), and Allophycocyanin- Cyanine 7 (APC- CY 7), conjugates are supplied as 100 tests in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 0.2 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD8 Section, Academic Press, New York, p. 149 2. Zamoyska, R. 1994. Immunity 1:243. |
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| Gene Symbol | CD8A |
| Gene Full Name | T-cell surface glycoprotein CD8 alpha chain |
| Alias Symbols | CD8, p32, Leu2 |
| NCBI Gene Id | 925 |
| Protein Name | T-cell surface glycoprotein CD8 alpha chain |
| Description of Target | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Uniprot ID | P01732 |
| Protein Accession # | NP_001139345.1 |
| Nucleotide Accession # | NM_001145873.1 |
