RayBio® G-Series Human Chemokine Antibody Array 1 Kit. Detects 38 Human Chemokines. Suitable for all liquid sample types.
Human Chemokine Array G1
Human Chemokine Array G1
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 38 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
BLC (CXCL13)
| CCL28 (MEC)
| Ck beta 8-1 (CCL23)
| CTACK (CCL27)
| CXCL16
|
ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
| I-309 (TCA-3/CCL1)
|
I-TAC (CXCL11)
| IL-8 (CXCL8)
| IP-10 (CXCL10)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
|
MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| MDC (CCL22)
| MIG (CXCL9)
|
MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
|
MPIF-1 (CCL23)
| NAP-2 (PPBP/CXCL7)
| PARC (CCL18)
| RANTES (CCL5)
| SDF-1 alpha (CXCL12 alpha)
|
SDF-1 beta (CXCL12 beta)
| TARC (CCL17)
| TECK (CCL25)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Chemokine Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-CHE-G1-4.gal)
GAL (GAL-AAH-CHE-G1-8.gal)
Excel (AAH-CHE-G1_Analysis_Tool.xls)
citations
Galectin-1-driven upregulation of SDF-1 in pancreatic stellate cells promotes pancreatic cancer metastasis
- Species:
- Human
- Sample Type:
- Conditioned Media (Cancer associated Pancreatic Stellate Cells)
- Year:
- 2017
- Journal Name:
- Cancer Letters
- PubMed ID:
- 28336327
Mononuclear-macrophages but not neutrophils act as major infiltrating anti-leptospiral phagocytes during leptospirosis.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2017
- Journal Name:
- Plos One
- PubMed ID:
- 28700741