AAH-CYT-5-4 | Human Cytokine Array C5 Biovalley

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Cytokine Array C5 Membrane Signals

Human Cytokine Array C5

Human Cytokine Array C5 is a membrane-based multiplex antibody array for simultaneous screening of 80 cytokines with ELISA-level specificity and pg/ml sensitivity. Compatible with serum, plasma, lysates, and cell culture supernatants, it enables rapid cytokine profiling using a simple western blot-style workflow.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	80
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Shipping Type	Blue ice
Storage	-20°C

Introduction

The Human Cytokine Array C5 is a multiplex, membrane-based antibody array designed for simultaneous screening of 80 human cytokines from a single sample. This high-density cytokine profiling platform combines sandwich ELISA specificity with the multiplex capability of protein arrays, enabling rapid comparison of cytokine expression patterns across experimental conditions.

Unlike traditional single-target ELISA assays or complex bead-based multiplex platforms, the Cytokine Array C5 provides a simple western blot-like workflow that can be completed in one day with minimal optimization. The array is compatible with serum, plasma, cell culture supernatants, tissue lysates, and cell lysates, making it ideal for biomarker discovery, inflammation research, and pathway analysis.

Learn More About Cytokine Arrays

Product Features

Screen 80 cytokines simultaneously from a single sample
Requires no specialized multiplex instrumentation
Western blot-style workflow with minimal optimization
pg/mL sensitivity using sandwich immunoassay design
Compatible with diverse liquid sample types
Proven performance with hundreds of peer-reviewed citations

Target Names

Scroll over each target protein for more information
Angiogenin Gene Symbol: ANG RNASE5 Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 BCA1 BLC SCYB13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	CK beta 8-1 (CCL23) Gene Symbol: CCL23 MIP3 MPIF1 SCYA23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA
ENA-78 (CXCL5) Gene Symbol: CXCL5 ENA78 SCYB5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 SCYA11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: CCL24 MPIF2 SCYA24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 SCYA26 UNQ216/PRO242 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	FGF-4 Gene Symbol: FGF4 HST HSTF1 KS3 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA
FGF-6 Gene Symbol: FGF6 HST2 HSTF2 Gene ID: 2251 Accession #: P10767 ELISA Kit: RayBio Human FGF-6 ELISA	FGF-7 (KGF) Gene Symbol: FGF7 EGK_17479 Gene ID: 2252 Accession #: P21781 ELISA Kit: RayBio Human FGF-7 ELISA	FGF-9 Gene Symbol: FGF9 Gene ID: 2254 Accession #: P31371 ELISA Kit: RayBio Human FGF-9 ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Gene ID: 2323 Accession #: P49771 ELISA Kit: RayBio Human Flt-3 L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 FKN NTT SCYD1 A-152E5.2 Gene ID: 6376 Accession #: P78423 ELISA Kit: RayBio Human Fractalkine ELISA
GCP-2 (CXCL6) Gene Symbol: CXCL6 GCP2 SCYB6 Gene ID: 6372 Accession #: P80162 ELISA Kit: RayBio Human GCP-2 ELISA	GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905 ELISA Kit: RayBio Human GDNF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	GRO alpha (CXCL1) Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA
GRO alpha/beta/gamma Accession #: P09341/P19875/P19876	HGF Gene Symbol: MET Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 SCYA1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA
IGFBP-1 Gene Symbol: IGFBP1 IBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 IBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 IBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-2 Gene Symbol: IL2 IL-2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: RayBio Human IL-3 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA
IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40/p70 Gene Symbol: IL12A NKSF1 Gene ID: 3592 Accession #: P29459
IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IL-16 Gene Symbol: IL16 Gene ID: 3603 Accession #: Q14005 ELISA Kit: RayBio Human IL-16 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	Leptin Gene Symbol: LEP OB OBS Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA
LIF Gene Symbol: LIF HILDA Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA	LIGHT (TNFSF14) Gene Symbol: TNFSF14 HVEML LIGHT UNQ391/PRO726 Gene ID: 8740 Accession #: O43557 ELISA Kit: RayBio Human LIGHT ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 MCP2 SCYA10 SCYA8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 MCP3 SCYA6 SCYA7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA
MCP-4 (CCL13) Gene Symbol: CCL13 MCP4 NCC1 SCYA13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 MDC SCYA22 A-152E5.1 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA	MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA
MIP-1 beta (CCL4) Gene ID: 388372; 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta (CCL15) Gene Symbol: CCL15 MIP5 NCC3 SCYA15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 LARC MIP3A SCYA20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	NAP-2 (PPBP/CXCL7) Gene Symbol: PPBP CTAP3 CXCL7 SCYB7 TGB1 THBGB1 Gene ID: 5473 Accession #: P02775 ELISA Kit: RayBio Human NAP-2 ELISA	NT-3 Gene Symbol: NTF3 Gene ID: 4908 Accession #: P20783 ELISA Kit: RayBio Human NT-3 ELISA
NT-4 Gene Symbol: NTF4 NTF5 Gene ID: 4909 Accession #: P34130 ELISA Kit: RayBio Human NT-4 ELISA	Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 BNSP OPN PSEC0156 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B OCIF OPG Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	PARC (CCL18) Gene Symbol: CCL18 AMAC1 DCCK1 MIP4 PARC SCYA18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA
PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	PLGF Gene Symbol: PGF PGFL PLGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA	RANTES (CCL5) Gene Symbol: CCL5 SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	SCF Gene Symbol: KITLG SCF Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 SDF1 SDF1A SDF1B Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA
TARC (CCL17) Gene Symbol: CCL17 SCYA17 TARC Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TGF beta 2 Gene Symbol: TGFB2 Gene ID: 7042 Accession #: P61812 ELISA Kit: RayBio Human TGF-beta 2 ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	Thrombopoietin (TPO) Gene Symbol: THPO MGDF Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA
TIMP-1 Gene Symbol: TIMP1 CLGI TIMP Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA TNFB TNFSF1 Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Suggested Application

Multiplex protein biomarker discovery
Cytokine expression profiling and screening
Inflammatory pathway and immune signaling studies
Drug response and translational research applications
Secretome analysis and cell communication research
Identification of disease-associated cytokine signatures

Typical Data

RayBiotech’s Human Cytokine Array C5 (AAH-CYT-5) was used to evaluate the cytokine response of bronchoalveolar lavage macrophages following stimulation with extracellular nucleosomes. Increased secretion of TNFα, IL-6, IL-10, CCL7, CCL8, CXCL9, and CXCL10 was observed 4H post stimulation. Figure adapted from Sripada et al., J Clin Invest, 2025.

RayBiotech’s Human Cytokine Antibody Array C5 (Cat# AAH-CYT-5) was used to profile SASP factors in conditioned media from WT and ATG7 KO valvular interstitial cells, with differential cytokine expression visualized as a heatmap. Figure adapted from Tang et al., Autophagy, 2025. .

AAH-CYT-5 was used to assess cytokine secretion in conditioned media from human dermal papilla cells, identifying reduced levels of IL-6, IL-8, MCP-1, and other senescence-associated factors following senolytic treatment. Figure adapted from Pappalardo et al., Aging Cell, 2025.

Cat# AAH-CYT-5 was used to assess cytokine secretion in conditioned media from human dermal fibroblasts, identifying osteoprotegerin (OPG) as a TGF-β1-induced factor associated with pro-fibrotic signaling. Figure adapted from McDowell-Sanchez et al., Biochemical and Biophysical Research Communications, 2025.

Cat# AAH-CYT-5 was used to assess cytokine secretion from macrophages exposed to tumor cell and mesenchymal stem cell conditioned media, identifying increased levels of CXCL1, CXCL5, and CXCL13 associated with stromal cell–driven immune modulation. Figure adapted from Zhang et al., Communications Biology, 2024.

Cat# AAH-CYT-5 was used to assess cytokine secretion in conditioned media from proliferating and senescent fibroblasts, identifying increased levels of BDNF, IL-6, CXCL1, HGF, and CXCL10 associated with the senescence-associated secretory phenotype. Figure adapted from Anerillas et al., Nature Communications, 2022.

Cat# AAH-CYT-5 was used to assess cytokine secretion from E. coli–activated human breast fibroblasts, identifying increased levels of inflammatory and pro-carcinogenic cytokines including IL-1β, IL-6, IL-8, MCP-1, and GRO family chemokines. Figure adapted from Alshehri et al., Cells, 2024.

Cat# AAH-CYT-5 was used to assess cytokine secretion in conditioned media from SKAP1-overexpressing colon cancer cells, identifying CXCL8 as the most strongly upregulated factor associated with neutrophil recruitment and tumor-promoting immune modulation. Figure adapted from Gao et al., Advanced Science, 2024.

Kit Components

Human Cytokine Antibody Array C5 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Datasheet (AAH-CYT-5.pdf)

SDS (AAH-CYT-5.pdf)

Excel (AAH-CYT-5_Analysis_Tool.xls)

citations

UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment
Year:
2024
Journal Name:
Nature Communications
PubMed ID:
38331898
A Facile Strategy for the Fabrication of Cell-Laden Porous Alginate Hydrogels based on Two-Phase Aqueous Emulsions
Species:
Human
Sample Type:
Human white adipose progenitors (WAPs) , Caco2 Cell
Year:
2023
Journal Name:
Advanced Functional Materials
Impairment of the NKT–STAT1–CXCL9 Axis Contributes to Vessel Fibrosis in Pulmonary Hypertension Caused by Lung Fibrosis
Species:
Human
Sample Type:
Peripheral blood mononuclear cells (PBMCs)
Year:
2022
Journal Name:
American Journal of Respiratory and Critical Care Medicine
A BDNF-TrkB autocrine loop enhances senescent cell viability
Species:
Human
Sample Type:
WI-38 fibroblasts
Year:
2022
Journal Name:
Nature Communications
Autophagy regulates cellular senescence by mediating the degradation of CDKN1A/p21 and CDKN2A/p16 through SQSTM1/p62-mediated selective autophagy in myxomatous mitral valve degeneration
Year:
2025
Journal Name:
Autophagy
PubMed ID:
39988732
Ephrin A1 Stimulates CCL2 Secretion to Facilitate Premetastatic Niche Formation and Promote Gastric Cancer Liver Metastasis
Year:
2025
Journal Name:
Cancer Research
PubMed ID:
39412948
Restoration of hair follicle inductive properties by depletion of senescent cells
Year:
2025
Journal Name:
Aging Cell
PubMed ID:
39614601
Human induced pluripotent stem cell-derived myotubes to model inclusion body myositis
Year:
2025
Journal Name:
Acta neuropathologica communications
PubMed ID:
39985015
Secreted Cytokines From Inflammatory Macrophages Modulate Sex Differences in Valvular Interstitial Cells on Hydrogel Biomaterials
Year:
2025
Journal Name:
Journal of Biomedical Materials Research Part A
PubMed ID:
39995146
Osteoprotegerin is induced by transforming growth factor-beta 1 and regulates pro-fibrotic responses in human dermal fibroblasts
Year:
2025
Journal Name:
Biochem Biophys Res Commun
PubMed ID:
40031101

Human Cytokine Array C5

Référence AAH-CYT-5-4

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Human Cytokine Array C5