RayBio® C-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C6
Human Cytokine Array C6
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 60 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| BDNF
| BLC (CXCL13)
| BMP-4
| BMP-6
|
Ck beta 8-1 (CCL23)
| CNTF
| EGF
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Eotaxin-3 (CCL26)
| FGF-6
| FGF-7 (KGF)
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GDNF
| GM-CSF
| I-309 (TCA-3/CCL1)
| IFN-gamma
|
IGFBP-1
| IGFBP-2
| IGFBP-4
| IGF-1
| IL-10
|
IL-13
| IL-15
| IL-16
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-1 ra (IL-1 F3)
| IL-2
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-7
| Leptin
| Light (TNFSF14)
| MCP-1 (CCL2)
|
MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
|
MIG (CXCL9)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
PARC (CCL18)
| PDGF-BB
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TGF beta 3
| TNF alpha
| TNF beta (TNFSF1B)
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C6 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
CTHRC1 drives megakaryocyte-mediated immunotherapy resistance in esophageal squamous cell carcinoma via the integrin αIIbβ3/Rap1 pathway and is targetable by mRNA vaccination
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant
- Year:
- 2026
- Journal Name:
- Molecular Cancer
- PubMed ID:
- 42169077
Circulating Metabolites Treat Human TMJ-OA by Eliminating Senescent Chondrocytes via the C1QBP/C1q/p14ARF Axis
- Species:
- Human
- Sample Type:
- Other Body Fluid (TMJ synovial fluid)
- Year:
- 2026
- Journal Name:
- Journal of Extracellular Vesicles
- PubMed ID:
- 41619174
Tumor neoantigen gene C7orf50 remodels the immune microenvironment by recruiting tumor-associated macrophages to promote hepatocellular carcinoma progression and lung metastasis
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (HCC cell culture supernatant)
- Year:
- 2026
- Journal Name:
- Journal for Immunotherapy of Cancer
- PubMed ID:
- 41592889
Novel stem cell therapy for cerebral palsy using stem cells from human exfoliated deciduous teeth
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (NSC-conditioned medium from co-culture with SHED, BMMSC or DF)
- Year:
- 2026
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 41578408
Dasatinib Remodels the Tumor Microenvironment and Sensitizes Small Cell Lung Cancer to Immunotherapy
- Year:
- 2025
- Journal Name:
- Cancer Research
- PubMed ID:
- 40712061
Nanobioreactor detection of space-associated hematopoietic stem and progenitor cell aging
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (nanobioreactor culture supernatants)
- Year:
- 2025
- Journal Name:
- Cell Stem Cell
- PubMed ID:
- 40912236
Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly
- Species:
- Human
- Sample Type:
- Other / Unspecified (cytokines secreted by ADSCs)
- Year:
- 2025
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 40595500
GPRC5A/CXCL8/NLRP3-mediated neutrophil extracellular traps drive gemcitabine-nab-paclitaxel resistance in pancreatic adenocarcinoma
- Species:
- Human
- Sample Type:
- Other / Unspecified (CM from GnP-resistant MIA PaCa-2 cells)
- Year:
- 2025
- Journal Name:
- Cancer Biology & Medicine
- PubMed ID:
- 40665634
Neuroplasticity therapy using glia-like cells derived from human mesenchymal stem cells for the recovery of cerebral infarction sequelae
- Year:
- 2025
- Journal Name:
- Molecular Therapy
- PubMed ID:
- 39563032
Endothelial Cells Offer a Better Option than Smooth Muscle Cells for the Treatment of Ischemic Limb Disease
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium from hiPSC-ECs, hiPSC-SMCs)
- Year:
- 2026
- Journal Name:
- Cells
- PubMed ID:
- 42738841
