RayBio® C-Series Human Cytokine Antibody Array 7 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C7
Human Cytokine Array C7
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 60 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Adiponectin (ACRP30)
| AgRP
| Angiopoietin-2
| Amphiregulin
| Axl
|
bFGF
| beta-NGF
| Betacellulin (BTC)
| CCL28 (MEC)
| CTACK (CCL27)
|
Dtk
| EGFR
| ENA-78 (CXCL5)
| Fas (TNFRSF6/Apo-1)
| FGF-4
|
FGF-9
| GCSF
| GITR Ligand (TNFSF18)
| GITR (TNFRSF18)
| GRO alpha/beta/gamma
|
GRO alpha (CXCL1)
| HCC-4 (CCL16)
| HGF
| ICAM-1 (CD54)
| ICAM-3 (CD50)
|
IGFBP-3
| IGFBP-6
| IGF-1 R
| IL-1 R4 (ST2)
| IL-1 R1
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-17A
| IL-2 R alpha
|
IL-6 R
| IL-8 (CXCL8)
| I-TAC (CXCL11)
| Lymphotactin (XCL1)
| MIF
|
MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-3 beta (CCL19)
| MSP alpha/beta
| NT-4
|
Osteoprotegerin (TNFRSF11B)
| Oncostatin M
| PLGF
| gp130
| TNF RII (TNFRSF1B)
|
TNF RI (TNFRSF1A)
| TECK (CCL25)
| TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VEGF-A
| VEGF-D
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C7 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
ICAM-1-suPAR-CD11b Axis Is a Novel Therapeutic Target for Metastatic Triple-Negative Breast Cancer
- Species:
- Human
- Sample Type:
- MDA-MB-231 cell
- Year:
- 2023
- Journal Name:
- Cancers
The Secretome of Aged Fibroblasts Promotes EMT-Like Phenotype in Primary Keratinocytes from Elderly Donors through BDNF-TrkB Axis
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2020
- Journal Name:
- Journal of Investigative Dermatology
Vasopressin Receptor Type-2 Mediated Signaling in Renal Cell Carcinoma Stimulates Stromal Fibroblast Activation
- Species:
- Human
- Sample Type:
- Caki1 cell
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection
- Species:
- Human
- Sample Type:
- Extracellular Vesicles (plasma Extracellular vesicles of HIV-NEF and ADAM17 chronic HIV patients)
- Year:
- 2016
- Journal Name:
- EBioMedicine
IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
- Species:
- Human
- Sample Type:
- Conditioned Media (Mammosphere breast cancer model)
- Year:
- 2015
- Journal Name:
- Nature Communications
- PubMed ID:
- 26503059
Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (HPS, Hypoxic PS1 supernates)
- Year:
- 2020
- Journal Name:
- Biomedicines
Novel human-derived extracellular matrix induces in vitro and in vivo vascularization and inhibits fibrosis
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Placental matrix)
- Year:
- 2015
- Journal Name:
- Biomaterials
- PubMed ID:
- 25725553
Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21
- Species:
- Human
- Sample Type:
- Cell Lysate (Colony Foruming Unit Endothelial Cells)
- Year:
- 2014
- PubMed ID:
- 24939570
Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling
- Species:
- Human
- Sample Type:
- Conditioned Media (Cancer Associated Fibroblasts and Lung cancer stem cells)
- Year:
- 2014
- Journal Name:
- Nature Communications
Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis
- Species:
- Human
- Sample Type:
- Conditioned Media (mammary epithelial cells, control and treated)
- Year:
- 2013
- Journal Name:
- Cancer Research