RayBio® C-Series Human Cytokine Antibody Array 8 Kit. Detects 54 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C8
Human Cytokine Array C8
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 54 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Activin A
| ALCAM (CD166)
| CD80 (B7-1)
| BMP-5
| BMP-7
|
Cardiotrophin-1 (CT-1)
| CD14
| CXCL16
| DR6 (TNFRSF21)
| Endoglin (CD105)
|
ErbB3
| E-Selectin
| Fas Ligand (TNFSF6)
| ICAM-2 (CD102)
| IGF-2
|
IL-1 R2
| IL-10 R beta
| IL-13 R alpha 2
| IL-18 BP alpha
| IL-18 R beta (AcPL)
|
MMP-3
| IL-2 R beta (CD122)
| IL-2 R gamma (Common gamma Chain)
| IL-21 R
| IL-5 R alpha
|
IL-9
| IP-10 (CXCL10)
| LAP/TGF beta 1
| Leptin R
| LIF
|
L-Selectin (CD62L)
| M-CSF R
| MMP-1
| MMP-13
| MMP-9
|
MPIF-1 (CCL23)
| NGFR (TNFRSF16)
| PDGF-AA
| PDGF-AB
| PDGF R alpha
|
PDGF R beta
| PECAM-1 (CD31)
| Prolactin
| SCF R (CD117/c-kit)
| SDF-1 beta (CXCL12 beta)
|
Siglec-5 (CD170)
| TGF alpha
| TGF beta 2
| Tie-1
| Tie-2
|
TIMP-4
| VE-Cadherin (CDH5)
| VEGFR2
| VEGFR3
| |
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C8 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Epigenetic regulation by HDAC11-driven STAT3 activation promotes pro-inflammatory cytokine production and endothelial dysfunction upon bacterial infection in diabetes
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture supernatants from iPSC-derived endothelial cells iPS-ECs)
- Year:
- 2026
- Journal Name:
- Cell Communication and Signaling
- PubMed ID:
- 41668160
HDAC11-Driven STAT3 Activation Modulates Epigenetic Regulation, Promoting Pro-Inflammatory Cytokine Production and Endothelial Dysfunction in Diabetes
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants collected 48 h post-infection)
- Year:
- 2025
- Journal Name:
- Research Square
It is not waste if it is therapy: cellular, secretory and functional properties of reamer-irrigator-aspirator (RIA)-derived autologous bone grafts
- Species:
- Human
- Sample Type:
- Other / Unspecified (liquid RIA samples)
- Year:
- 2025
- Journal Name:
- Journal of orthopaedics and traumatology : official journal of the Italian Society of Orthopaedics and Traumatology
- PubMed ID:
- 40140186
Renal cancer secretome induces migration of mesenchymal stromal cells
- Species:
- Human
- Sample Type:
- mesenchymal stem/stromal cells (MSCs)
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy
Discovery of a transdermally deliverable pentapeptide for activating AdipoR1 to promote hair growth
- Species:
- Human
- Sample Type:
- Serum (concentrated serum-free medium from human DP cells)
- Year:
- 2021
- Journal Name:
- EMBO Molecular Medicine
- PubMed ID:
- 34486824
Tumor specific cytokine pattern explored in renal cell carcinoma
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (tissue homogenates normal, para-cancerous and tumor RCC tissue)
- Year:
- 2026
- Journal Name:
- PLoS ONE
- PubMed ID:
- 42809553
Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment
- Species:
- Human
- Sample Type:
- Other / Unspecified (secreted media from A375M2, A375P melanoma cells)
- Year:
- 2019
- Journal Name:
- Cell
- PubMed ID:
- 30712866
Notch3 promotes prostate cancer-induced bone lesion development via MMP-3
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (lysates of subcutaneous, tibial tumors)
- Year:
- 2020
- Journal Name:
- Oncogene
- PubMed ID:
- 31467432
Xenografting of human umbilical mesenchymal stem cells from Wharton's jelly ameliorates mouse spinocerebellar ataxia type 1
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2019
- Journal Name:
- Translational neurodegeneration
- PubMed ID:
- 31508229
Reversal of bleomycin-induced rat pulmonary fibrosis by a xenograft of human umbilical mesenchymal stem cells from Wharton's jelly
- Species:
- Human
- Sample Type:
- Tissue Lysate (mesenchymal tissue from human umbilical cords)
- Year:
- 2019
- Journal Name:
- Theranostics
