RayBio® C-Series Human Cytokine Antibody Array 9 Kit. Detects 51 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C9
Human Cytokine Array C9
Contact Us for Bulk Request
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 51 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Adipsin (Complement Factor D)
| BCAM
| CD30 (TNFRSF8)
| CD40 (TNFRSF5)
| Fc gamma RIIB/C (CD32B/C)
|
Ferritin
| FLRG
| Follistatin
| Furin
| Galectin-7
|
GDF-15
| Growth Hormone
| IL-10 R alpha
| IL-22
| IL-28A (IFN-lambda 2)
|
IL-29 (IFN-lambda 1)
| IL-31
| Insulin
| Luteinizing hormone
| LIMPII
|
LYVE-1
| Marapsin
| MICA
| MICB
| MMP-2
|
MMP-7
| MMP-8
| MMP-10
| NCAM-1 (CD56)
| Nidogen-1
|
NrCAM
| NRG1-beta 1 (HRG1-beta 1)
| Osteopontin (SPP1)
| PAI-1
| Platelet factor 4 (CXCL4)
|
PSA-total
| RAGE
| RANK (TNFRSF11A)
| Resistin
| SAA (Serum Amyloid A)
|
Siglec-9
| TACE
| TIM-1 (KIM-1)
| TRAIL R2 (TNFRSF10B/DR5)
| Trappin-2
|
TREM-1
| TSH
| TSLP
| VCAM-1 (CD106)
| VEGF-C
|
XEDAR
| ||||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C9 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Renal cancer secretome induces migration of mesenchymal stromal cells
- Species:
- Human
- Sample Type:
- mesenchymal stem/stromal cells (MSCs)
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy
Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment
- Species:
- Human
- Sample Type:
- Other / Unspecified (secreted media from A375M2, A375P melanoma cells)
- Year:
- 2019
- Journal Name:
- Cell
- PubMed ID:
- 30712866
Stromal-derived interleukin 6 drives epithelial-to-mesenchymal transition and therapy resistance in esophageal adenocarcinoma
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (081RF fibroblast supernatant)
- Year:
- 2019
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- PubMed ID:
- 30670657
Paracrine Induction of Epithelial-Mesenchymal Transition Between Colorectal Cancer Cells and its Suppression by a p53/miR-192/215/NID1 Axis
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium of CRC cell lines)
- Year:
- 2019
- Journal Name:
- Cellular and Molecular Gastroenterology and Hepatology
- PubMed ID:
- 30831320
Glyceryl trinitrate‑induced cytotoxicity of docetaxel‑resistant prostatic cancer cells is associated with differential regulation of clusterin
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture supernatants of DU145, DU145-DR, PC3 AG, PC3-D12 cells)
- Year:
- 2019
- Journal Name:
- International Journal of Oncology
- PubMed ID:
- 30720069
Innate, non-cytolytic CD8+ T cell-mediated suppression of HIV replication by MHC-independent inhibition of virus transcription
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2020
- Journal Name:
- Plos Pathogens
Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (HPS, Hypoxic PS1 supernates)
- Year:
- 2020
- Journal Name:
- Biomedicines
Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling
- Species:
- Human
- Sample Type:
- Conditioned Media (Cancer Associated Fibroblasts and Lung cancer stem cells)
- Year:
- 2014
- Journal Name:
- Nature Communications
Comparison of cytokine/chemokine levels in aqueous humor of primary open-angle glaucoma patients with positive or negative outcome following trabeculectomy
- Species:
- Human
- Sample Type:
- Other Body Fluid (aqueous humor)
- Year:
- 2019
- Journal Name:
- Bioscience Reports
- PubMed ID:
- 30967499
The effect of platelet lysate fibrinogen on the functionality of MSCs in immunotherapy.
- Species:
- Human
- Sample Type:
- Platelet Poor Plasma (unfractionated platelet lysates (uPL) and fibrinogen depleted platelet lysate (fdPL) - effect of platelet lysate fibrinogen on the functionality of MSCs in immunotherapy); Serum (effect of platelet lysate fibrinogen on the functionality of MSCs in immunotherapy)
- Year:
- 2013
- Journal Name:
- Biomaterials
- PubMed ID:
- 23891515
