RayBio® G-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array G6 & G7. Detects 120 Human Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 120 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Adiponectin (ACRP30)
| AgRP
| Amphiregulin
| Angiogenin
| Angiopoietin-2
|
Axl
| BDNF
| bFGF
| BLC (CXCL13)
| BMP-4
|
BMP-6
| beta-NGF
| Betacellulin (BTC)
| CCL28 (MEC)
| CK beta 8-1 (CCL23)
|
CNTF
| CTACK (CCL27)
| Dtk
| EGF
| EGFR
|
ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| Fas (TNFRSF6/Apo-1)
|
FGF-4
| FGF-6
| FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
|
Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
| GCSF
| GDNF
| GITR (TNFRSF18)
|
GITR Ligand (TNFSF18)
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
|
HGF
| I-309 (TCA-3/CCL1)
| ICAM-1 (CD54)
| ICAM-3 (CD50)
| IFN-gamma
|
IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IGFBP-6
|
IGF-1
| IGF-1 R
| IL-1 R4 (ST2)
| IL-1 R1
| IL-10
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-13
| IL-15
|
IL-16
| IL-17A
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
IL-2
| IL-2 R alpha
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-6 R
| IL-7
| IL-8 (CXCL8)
| I-TAC (CXCL11)
|
Leptin
| Light (TNFSF14)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
|
MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
| MIF
|
MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
|
MIP-3 beta (CCL19)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| NT-3
| NT-4
|
Oncostatin M
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
| PDGF-BB
| PLGF
|
RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
| gp130
| TNF RII (TNFRSF1B)
|
TNF RI (TNFRSF1A)
| TARC (CCL17)
| TECK (CCL25)
| TGF beta 3
| TGF beta 1
|
Thrombopoietin (TPO)
| TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VEGF-A
| VEGF-D
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Human Cytokine Antibody Array G1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-CYT-G6-4.gal)
GAL (GAL-AAH-CYT-G6-8.gal)
GAL (GAL-AAH-CYT-G7-4.gal)
GAL (GAL-AAH-CYT-G7-8.gal)
Excel (AAH-CYT-G6_Analysis_Tool.xls)
Excel (AAH-CYT-G7_Analysis_Tool.xls)
citations
CCL4 and MIF: Prognostic Biomarkers for Evaluating the Chemoradiotherapy Response and Prognosis in Patients with ESCC
- Species:
- Human
- Sample Type:
- Serum (pretreatment ESCC patients)
- Year:
- 2025
- Journal Name:
- Journal of Cancer
- PubMed ID:
- 40092701
Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (Cal27 cells, LY2835219 ± metformin)
- Year:
- 2020
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 33116117
Cancer-associated fibroblasts promote prostate tumor growth and progression through upregulation of cholesterol and steroid biosynthesis
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (CAF spheroid-conditioned medium)
- Year:
- 2020
- Journal Name:
- Cell Communication and Signaling
- PubMed ID:
- 31980029
Interleukin-6 derived from cancer-associated fibroblasts attenuates the p53 response to doxorubicin in prostate cancer cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media from CAFs or LNCaP cells)
- Year:
- 2020
- Journal Name:
- Cell Death Discovery
- PubMed ID:
- 32528731
CCL2/CCL5 secreted by the stroma induce IL-6/PYK2 dependent chemoresistance in ovarian cancer
- Species:
- Human
- Sample Type:
- Conditioned Media (Stroma and Ovarian Cancer Model); Conditioned media (mesenchymal cells from ascites of a patient with Stage III serous adenocarcinoma)
- Year:
- 2018
- Journal Name:
- Molecular Cancer
- PubMed ID:
- 29455640
MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation
- Species:
- Human
- Sample Type:
- Conditioned Media (Senescence model with Fibroblasts)
- Year:
- 2015
- Journal Name:
- Nature Cell Biology
- PubMed ID:
- 26147250
Predictive value of EGF and uPAR for chemoradiotherapy response and survival in patients with esophageal squamous cell carcinoma
- Species:
- Human
- Sample Type:
- Serum (before treatment and at 40 gy)
- Year:
- 2020
- Journal Name:
- Annals of translational medicine
- PubMed ID:
- 33241001
Tumourmicro-environment elicits innate resistance to RAF inhibitors through HGF secretion
- Species:
- Human
- Sample Type:
- Conditioned Media (Stromal Cell Lines)
- Year:
- 2012
- Journal Name:
- Nature
- PubMed ID:
- 22763439
Endothelial Cells Promote Docetaxel Resistance of Prostate Cancer Cells by Inducing ERG Expression and Activating Akt/mTOR Signaling Pathway
- Species:
- Human
- Sample Type:
- Serum (serum-free media from prostate cancer cell culture, HUVEC, prostate cancer cell…)
- Year:
- 2020
- Journal Name:
- Frontiers in Oncology
- PubMed ID:
- 33425737
Glucocorticoids Suppress Selected Components of the Senescence-Associated Secretory Phenotype
- Species:
- Human
- Sample Type:
- Conditioned Media (HCA2 (foreskin), IMR-90 (fetal lung fibroblasts), and T47D (breast cancer))
- Year:
- 2012
- Journal Name:
- Aging Cell


