Quantibody® Human Cytokine Array 1000 Kit. A combination of Human Inflammation Array Q3 and Human Growth Factor Array Q1. Detects 80 Human Inflammatory and Growth Factors. Suitable for all liquid sample types.
Human Cytokine Array Q1000
Human Cytokine Array Q1000
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 80 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application
|
Related Products
Human Inflammation Array Q3
As low as $840.00
Human Growth Factor Array Q1
As low as $840.00
Human Cytokine Array C1000
As low as $650.00
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 100 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Amphiregulin
| BDNF
| beta-NGF
| bFGF
| BLC (CXCL13)
|
BMP-4
| BMP-5
| BMP-7
| EGF
| EGFR
|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| FGF-4
| FGF-7 (KGF)
| GCSF
|
GDF-15
| GDNF
| GM-CSF
| Growth Hormone
| EG-VEGF (PK1)
|
HB-EGF
| HGF
| I-309 (TCA-3/CCL1)
| ICAM-1 (CD54)
| IFN-gamma
|
IGF-1
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-6
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
| IL-10
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-13
| IL-15
|
IL-16
| IL-17A
| IL-2
| IL-4
| IL-5
|
IL-6
| IL-6 R
| IL-7
| IL-8 (CXCL8)
| Insulin
|
MCP-1 (CCL2)
| M-CSF
| M-CSF R
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| NGFR (TNFRSF16)
| NT-3
| NT-4
|
Osteoprotegerin (TNFRSF11B)
| PDGF-AA
| PDGF-BB
| PLGF
| RANTES (CCL5)
|
SCF
| SCF R (CD117/c-kit)
| TGF alpha
| TGF beta 1
| TGF beta 3
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
|
TNF RII (TNFRSF1B)
| VEGF-A
| VEGF-D
| VEGFR2
| VEGFR3
|
Species Detected
Human
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Human Cytokine Array Q1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-INF-3.gal)
GAL (GAL-QAH-GF-1.gal)
Excel (QAH-INF-3_Analysis_Tool.xls)
Excel (QAH-GF-1_Analysis_Tool.xls)
citations
Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton
- Year:
- 2026
- Journal Name:
- Communications Biology
- PubMed ID:
- 41781748
PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatant from Cal-27 cells, hOMK cells)
- Year:
- 2024
- Journal Name:
- Cancer Cell International
- PubMed ID:
- 38169376
Protective effects of conditioned media of immortalized stem cells from human exfoliated deciduous teeth on pressure ulcer formation
- Species:
- Human
- Sample Type:
- human exfoliated deciduous teeth (SHED) stem cell
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Assessing Bioprinted Functionalized Grafts for Biological Tendon Augmentation In Vitro
- Species:
- Human
- Sample Type:
- Other / Unspecified (factors released by tenocytes within hydrogel formulations)
- Year:
- 2024
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 38731971
The Different Immune Responses by Age Are due to the Ability of the Fetal Immune System to Secrete Primal Immunoglobulins Responding to Unexperienced Antigens
- Species:
- Human
- Sample Type:
- Extracellular Vesicles (EVs)
- Year:
- 2021
- Journal Name:
- International Journal of Biological Sciences
- PubMed ID:
- 35002513
Retention of Key Characteristics of Unprocessed Chorion Tissue Resulting in a Robust Scaffold to Support Wound Healing
- Species:
- Human
- Sample Type:
- Hypothermically stored chorion membrane (HSCM)
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Specific features of ex-obese patients significantly influence the functional cell properties of adipose-derived stromal cells
- Species:
- Human
- Sample Type:
- Adipose-derived stromal cells (ADSC)
- Year:
- 2022
- Journal Name:
- Journal of Cellular and Molecular Medicine
Type I and II Diabetic Adipose-Derived Stem Cells Respond In Vitro to Dehydrated Human Amnion/Chorion Membrane Allograft Treatment by Increasing Proliferation, Migration, and Altering Cytokine Secretion
- Species:
- Human
- Sample Type:
- Conditioned Media (Adipose derived stem cells with or without dHCAM)
- Year:
- 2016
- Journal Name:
- Advances in Wound Care
- PubMed ID:
- 26862462
Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro
- Species:
- Human
- Sample Type:
- Conditioned Media (ADCS, BM-MSCs and HSCs stem cell lines)
- Year:
- 2015
- Journal Name:
- Journal of Biomedical Materials Research Part B: Applied Biomaterials
- PubMed ID:
- 26175122
A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression
- Species:
- Human
- Sample Type:
- Conditioned Media (MSC, umbilical and bone marrow stem cell cultures)
- Year:
- 2015
- Journal Name:
- Stem Cells and Development
- PubMed ID:
- 25758779