Quantibody® Human Cytokine Array 1000 Kit. A combination of Human Inflammation Array Q3 and Human Growth Factor Array Q1. Detects 80 Human Inflammatory and Growth Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 80 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application
|
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 100 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Amphiregulin
| BDNF
| beta-NGF
| bFGF
| BLC (CXCL13)
|
BMP-4
| BMP-5
| BMP-7
| EGF
| EGFR
|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| FGF-4
| FGF-7 (KGF)
| GCSF
|
GDF-15
| GDNF
| GM-CSF
| Growth Hormone
| EG-VEGF (PK1)
|
HB-EGF
| HGF
| I-309 (TCA-3/CCL1)
| ICAM-1 (CD54)
| IFN-gamma
|
IGF-1
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
IGFBP-6
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
| IL-10
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-13
| IL-15
|
IL-16
| IL-17A
| IL-2
| IL-4
| IL-5
|
IL-6
| IL-6 R
| IL-7
| IL-8 (CXCL8)
| Insulin
|
MCP-1 (CCL2)
| M-CSF
| M-CSF R
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| NGFR (TNFRSF16)
| NT-3
| NT-4
|
Osteoprotegerin (TNFRSF11B)
| PDGF-AA
| PDGF-BB
| PLGF
| RANTES (CCL5)
|
SCF
| SCF R (CD117/c-kit)
| TGF alpha
| TGF beta 1
| TGF beta 3
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
|
TNF RII (TNFRSF1B)
| VEGF-A
| VEGF-D
| VEGFR2
| VEGFR3
|
Species Detected
Human
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Human Cytokine Array Q1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-INF-3.gal)
GAL (GAL-QAH-GF-1.gal)
Excel (QAH-INF-3_Analysis_Tool.xls)
Excel (QAH-GF-1_Analysis_Tool.xls)
citations
Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton
- Year:
- 2026
- Journal Name:
- Communications Biology
- PubMed ID:
- 41781748
PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatant from Cal-27 cells, hOMK cells)
- Year:
- 2024
- Journal Name:
- Cancer Cell International
- PubMed ID:
- 38169376
Protective effects of conditioned media of immortalized stem cells from human exfoliated deciduous teeth on pressure ulcer formation
- Species:
- Human
- Sample Type:
- human exfoliated deciduous teeth (SHED) stem cell
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Assessing Bioprinted Functionalized Grafts for Biological Tendon Augmentation In Vitro
- Species:
- Human
- Sample Type:
- Other / Unspecified (factors released by tenocytes within hydrogel formulations)
- Year:
- 2024
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 38731971
The Different Immune Responses by Age Are due to the Ability of the Fetal Immune System to Secrete Primal Immunoglobulins Responding to Unexperienced Antigens
- Species:
- Human
- Sample Type:
- Extracellular Vesicles (EVs)
- Year:
- 2021
- Journal Name:
- International Journal of Biological Sciences
- PubMed ID:
- 35002513
Retention of Key Characteristics of Unprocessed Chorion Tissue Resulting in a Robust Scaffold to Support Wound Healing
- Species:
- Human
- Sample Type:
- Hypothermically stored chorion membrane (HSCM)
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Specific features of ex-obese patients significantly influence the functional cell properties of adipose-derived stromal cells
- Species:
- Human
- Sample Type:
- Adipose-derived stromal cells (ADSC)
- Year:
- 2022
- Journal Name:
- Journal of Cellular and Molecular Medicine
Type I and II Diabetic Adipose-Derived Stem Cells Respond In Vitro to Dehydrated Human Amnion/Chorion Membrane Allograft Treatment by Increasing Proliferation, Migration, and Altering Cytokine Secretion
- Species:
- Human
- Sample Type:
- Conditioned Media (Adipose derived stem cells with or without dHCAM)
- Year:
- 2016
- Journal Name:
- Advances in Wound Care
- PubMed ID:
- 26862462
Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro
- Species:
- Human
- Sample Type:
- Conditioned Media (ADCS, BM-MSCs and HSCs stem cell lines)
- Year:
- 2015
- Journal Name:
- Journal of Biomedical Materials Research Part B: Applied Biomaterials
- PubMed ID:
- 26175122
A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression
- Species:
- Human
- Sample Type:
- Conditioned Media (MSC, umbilical and bone marrow stem cell cultures)
- Year:
- 2015
- Journal Name:
- Stem Cells and Development
- PubMed ID:
- 25758779






