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Human E-Selectin ELISA Kit
Human E-Selectin ELISA Kit
RayBio® Human E-Selectin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 30 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 30 pg/ml - 18000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 50 - 200 fold |
| Accession Number | P16581 |
| Gene Id | 6401 |
| Gene Symbols | SELE, ELAM1 |
| Protein Name / Synonyms | E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human E-Selectin qIPCR ELISA Kit
As low as $749.00
RayPlex® Human E-selectin Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human E-Selectin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified E-Selectin (CD62E / SELE / ELAM-1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
TNF-α and E-Selectin as Valuable Biomarkers in Patients with Acute Coronary Artery Syndrome
- Species:
- Human
- Sample Type:
- Serum (acute coronary syndrome patients)
- Year:
- 2025
- Journal Name:
- International Journal of Molecular and Cellular Medicine
- PubMed ID:
- 40765755
Uremic serum damages endothelium by provoking excessive neutrophil extracellular trap formation
- Species:
- Human
- Sample Type:
- Plasma (uremic patients)
- Year:
- 2021
- Journal Name:
- Scientific Reports
- PubMed ID:
- 34728714
Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous Growth
- Species:
- Human
- Sample Type:
- Conditioned media (tissue culture)
- Year:
- 2019
- Journal Name:
- Cells
Biomarkers of cardiometabolic complications in survivors of childhood ac
- Species:
- human
- Sample Type:
- Plasma
- Year:
- 2020
- Journal Name:
- Scientific reports
- PubMed ID:
- 33299020
CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease
- Species:
- Human
- Sample Type:
- Plasma (Kawasaki's Disease patients)
- Year:
- 2015
- Journal Name:
- Circulation Research
- PubMed ID:
- 25605650
Comparison of Two Ginkgo biloba L. Extracts on Oxidative Stress and Inflammation Markers in Human Endothelial Cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture medium of HUVECs)
- Year:
- 2019
- Journal Name:
- Mediators of Inflammation
- PubMed ID:
- 31341420
Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism
- Species:
- Human
- Sample Type:
- Conditioned Media (Human retinal capillary endothelial and human umbilical vein endothelial cells); human retinal capillary endothelial cells (HRCEC)
- Year:
- 2023
- Journal Name:
- Molecular and Cellular Biology
The association of biomarkers with pain and function in acute and subacute low back pain: a secondary analysis of an RCT
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Bmc Musculoskeletal Disorders
Increased ICAM, VCAM, and E-selectin levels in first manic episode
- Species:
- Human
- Sample Type:
- Plasma (Mania Patients)
- Year:
- 2014
- Journal Name:
- Cells
Effect of soy protein and isoflavones on blood pressure and endothelial cytokines: a 6-month randomized controlled trial among postmenopausal women.
- Species:
- Human
- Sample Type:
- Serum (postmenopausal women with pre or early diabetes w)
- Year:
- 2013
- Journal Name:
- Journal of Hypertension
- PubMed ID:
- 23203140
