RayBio® C-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
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Human Growth Factor Array C1
Human Growth Factor Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 41 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Amphiregulin
| bFGF
| beta-NGF
| EGF
| EGFR
|
| FGF-4
| FGF-6
| FGF-7 (KGF)
| GCSF
| GDNF
|
| GM-CSF
| HB-EGF
| HGF
| IGFBP-1
| IGFBP-2
|
| IGFBP-3
| IGFBP-4
| IGFBP-6
| IGF-1
| IGF-1 R
|
| IGF-2
| M-CSF
| M-CSF R
| NT-3
| NT-4
|
| PDGF R alpha
| PDGF R beta
| PDGF-AA
| PDGF-AB
| PDGF-BB
|
| PLGF
| SCF
| SCF R (CD117/c-kit)
| TGF alpha
| TGF beta 1
|
| TGF beta 2
| TGF beta 3
| VEGF-A
| VEGFR2
| VEGFR3
|
| VEGF-D
| ||||
Typical Data
Kit Components
- Human Growth Factor Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Reconstruction of the human nipple-areolar complex: a tissue engineering approach
- Year:
- 2024
- Journal Name:
- Frontiers in bioengineering and biotechnology
- PubMed ID:
- 38425730
A novel role of CD73-IFNγ signalling axis in human mesenchymal stromal cell mediated inflammatory macrophage suppression
- Year:
- 2024
- Journal Name:
- Regenerative therapy
- PubMed ID:
- 38845846
Paracrine signals influence patterns of fibrocartilage differentiation in a lyophilized gelatin hydrogel for applications in rotator cuff repair
- Year:
- 2024
- Journal Name:
- Biomater Sci
- PubMed ID:
- 39150417
Transplantable stem cell nanobridge scaffolds for accelerating articular cartilage regeneration
- Species:
- Human
- Sample Type:
- Human adipose tissue-derived mesenchymal stem cells (hMSC)
- Year:
- 2023
- Journal Name:
- Biomaterials
Viscoelastic hydrogels for interrogating pancreatic cancer-stromal cell interactions
- Year:
- 2023
- Journal Name:
- Materials Today Bio
Scutellarin suppresses the metastasis of triple-negative breast cancer via targeting TNFα/TNFR2-RUNX1-triggered G-CSF expression in endothelial cells
- Species:
- Human
- Sample Type:
- Human mammary microvascular endothelial cells (HMMECs)
- Year:
- 2023
- Journal Name:
- Biochemical Pharmacology
Vessel-Derived Decellularized Extracellular Matrices (VdECM): Novel Bio-Engineered Materials for the Wound Healing
- Species:
- Human
- Sample Type:
- adipocyte-derived stem cell (ASC)
- Year:
- 2023
- Journal Name:
- Tissue Engineering and Regenerative Medicine
Comparison of the Cost and Effect of Combined Conditioned Medium and Conventional Medium for Fallopian Tube Organoid Cultures
- Species:
- Human
- Sample Type:
- human umbilical vein endothelial cells (HUVEC)
- Year:
- 2023
- Journal Name:
- Cell Transplantation
MDA-9/Syntenin in the tumor and microenvironment defines prostate cancer bone metastasis
- Year:
- 2023
- Journal Name:
- Proc Natl Acad Sci U S A
- PubMed ID:
- 37922327
Harnessing the synergy of perfusable muscle flap matrix and adipose-derived stem cells for prevascularization and macrophage polarization to reconstruct volumetric muscle loss
- Species:
- Human
- Sample Type:
- human adipose-derived stem cells (hASCs)
- Year:
- 2022
- Journal Name:
- Bioactive materials