Human IL-13 Antibody : LE/AF
Référence OASB01230
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Human IL-13 Antibody : LE/AF (OASB01230)
| Datasheets/Manuals | Printable datasheet for Human IL-13 Antibody : LE/AF (OASB01230) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | JES10-5A2 |
| Isotype | IgG1 |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | ELISPOT, FC, IHC-F, ICC |
| Reconstitution and Storage | Store at -20C |
| Immunogen | COS-expressed human IL-13 |
| Concentration | 0.5 mg/mL |
| Specificity | IL-13 |
| Characterization | To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform tocharacteristics of a standard reference reagent. |
| Application Info | ELISA Capture: JES10-5A2 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels. 1, 2 Purified JES10-5A2 antibody should be paired with biotinylated JES2E10 antibody (OASB01232) as the detection antibody, with purified recombinant human IL-13 as the standard. Immunofluorescence/Flow Cytometry: JES10-5A2 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 (OASB012111) should be used as a specificity control. Western Blotting: The purified JES10-5A2 antibody is also useful for Western blotting applications. A concentration of 1-5ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, OASB01897) has been found to enable detection of <= 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form of JES10-5A2 can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 (OASB00095) should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. McKenzie, A., and G. Zurawski. 1994. Measurement of IL- 13. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.18 2. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP- labeled anti- cytokine antibodies. In CurrentProtocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit6.20. |
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| Gene Symbol | IL13 |
| Gene Full Name | Interleukin-13 |
| Alias Symbols | P600, IL-13 |
| NCBI Gene Id | 3596 |
| Protein Name | interleukin-13 |
| Description of Target | This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. |
| Uniprot ID | P35225 |
| Protein Accession # | NP_002179.2 |
| Nucleotide Accession # | NM_002188.2 |
