Human IL-7 Antibody : LE/AF
Référence OASB01225
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Human IL-7 Antibody : LE/AF (OASB01225)
| Datasheets/Manuals | Printable datasheet for Human IL-7 Antibody : LE/AF (OASB01225) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | BVD10-40F6 |
| Isotype | IgG1 |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | ICC |
| Reconstitution and Storage | Store at -20C |
| Immunogen | E. coli-expressed human IL-7 |
| Concentration | 0.5 mg/mL |
| Specificity | IL-7 |
| Characterization | To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform tocharacteristics of a standard reference reagent. |
| Application Info | ELISA Capture: BVD10-40F6 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying human IL-7 protein levels. 1, 2 Purified BVD10-40F6 antibody should be paired with biotinylated BVD10-11C10 antibody (OASB01227) as the detection antibody, with purified recombinant human IL-7 as the 3standard. Western Blotting: The purified BVD10-40F6 antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5ug/mL (in conjunction with ALPH-labeled Goat Anti-Rat-Ig, OASB01897) has been found to enable detection of <= 100 ng/lane of recombinant human IL-7 under reducing conditions. Neutralization: The LE/AF form of BVD10-40F6 can be used for neutralizing human IL-7 bioactivity. A suitable LE/AF Rat IgG1 (OASB00095) should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Abrams, J.S., M.- G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5 2. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP- labeled anti- cytokine antibodies. In CurrentProtocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit6.20. |
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| Gene Symbol | IL7 |
| Gene Full Name | Interleukin-7 |
| Alias Symbols | IL-7 |
| NCBI Gene Id | 3574 |
| Protein Name | interleukin-7 |
| Description of Target | The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. |
| Uniprot ID | P13232 |
| Protein Accession # | NP_000871.1 |
| Nucleotide Accession # | NM_000880.3 |
