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> Human IL-8 ELISA Kit

Human IL-8 ELISA Kit

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Human IL-8 ELISA Kit Standard CurveHuman IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA KitELH-IL8 was used to measure IL-8 in plasma and serum samples from patients with non-small cell lung cancer compared to normal individuals. Figure adapted from Lim et al., Diseases, 2024.ELH-IL8 was used to measure CXCL8 in conditioned media from tumour cell lines and extracellular vesicle–treated lymphatic endothelial cells. Figure adapted from Su et al., Journal of Extracellular Vesicles, 2023.ELH-IL8 was used to measure CXCL8 in colon cancer cell culture supernatants under SKAP1 overexpression or knockdown conditions. Figure adapted from Gao et al., Adv Sci (Weinh)., 2024.ELH-IL8 was used to measure IL-8 in culture supernatants of LPS-stimulated Caco-2 cells treated with bioaccessible fractions of pomegranate seed oils. Figure adapted from Banares et al., Food Research International, 2023.IL-8 levels in trophoblast cell culture supernatants were quantified using a RayBiotech Human IL-8 ELISA Kit to evaluate the effect of miR-95-3p and EPM2A modulation in severe preeclampsia. Figure adapted from Gao et al., Arch Biochem Biophys, 2023.ELH-IL8 was used to measure IL-8 in conditioned media from human mesenchymal stem cells, degenerative disc cells, and 1:1 co-cultures treated with o-Vanillin. Figure adapted from Li et al., Cells, 2022.Human IL-8 ELISA KitHuman IL-8 ELISA KitHuman IL-8 ELISA Kit5 Plate Kit Packaging (one box)

Human IL-8 ELISA Kit Standard Curve

Human IL-8 ELISA Kit

RayBio® Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit
SpeciesHuman
Sensitivity
1 pg/ml

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Detection Range
1 pg/ml - 600 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P10145
Gene Id
3576
Gene Symbols
CXCL8, IL8
Protein Name / Synonyms
Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Cancer, Inflammation, Antibody Drug Development, Protein Therapeutics, Chemokine Biology
Shipping TypeBlue ice
Storage≤-20°C

Target Background

Interleukin 8 (IL-8) is a chemotactic factor that attracts neutrophils, basophils and T-cells during an inflammatory process. It is released from a variety cell types such as monocytes, macrophages, neutrophils and intestine, kidney, placenta and bone marrow cells in response to inflammation. It is involved many cellular processes including cell proliferation, tissue remodeling and angiogenesis. IL-8 mediates its effects by binding to two heterotrimeric G protein-coupled receptors, CXCR1 and CXCR2. Given that it is a major mediator of inflammation, IL-8 was markedly increased in infants diagnosed with perinatal infection.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

ELH-IL8 was used to measure IL-8 in plasma and serum samples from patients with non-small cell lung cancer compared to normal individuals. Figure adapted from Lim et al., Diseases, 2024.

ELH-IL8 was used to measure CXCL8 in conditioned media from tumour cell lines and extracellular vesicle–treated lymphatic endothelial cells. Figure adapted from Su et al., Journal of Extracellular Vesicles, 2023.

ELH-IL8 was used to measure CXCL8 in colon cancer cell culture supernatants under SKAP1 overexpression or knockdown conditions. Figure adapted from Gao et al., Adv Sci (Weinh)., 2024.

ELH-IL8 was used to measure IL-8 in culture supernatants of LPS-stimulated Caco-2 cells treated with bioaccessible fractions of pomegranate seed oils. Figure adapted from Banares et al., Food Research International, 2023.

IL-8 levels in trophoblast cell culture supernatants were quantified using a RayBiotech Human IL-8 ELISA Kit to evaluate the effect of miR-95-3p and EPM2A modulation in severe preeclampsia. Figure adapted from Gao et al., Arch BiochemBiophys, 2023.

ELH-IL8 was used to measure IL-8 in conditioned media from human mesenchymal stem cells, degenerative disc cells, and 1:1 co-cultures treated with o-Vanillin. Figure adapted from Li et al., Cells, 2022.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

citations

  • Tumour extracellular vesicles induce neutrophil extracellular traps to promote lymph node metastasis

    Species:
    Human
    Sample Type:
    BEAS-2B cell, A549 cell
    Year:
    2023
    Journal Name:
    Journal of Extracellular Vesicles

  • Circulating Interleukins as Biomarkers in Non-Small Cell Lung Cancer Patients: A Pilot Study Compared to Normal Individuals

    Year:
    2024
    Journal Name:
    Diseases
    PubMed ID:
    39329890

  • SKAP1 Expression in Cancer Cells Enhances Colon Tumor Growth and Impairs Cytotoxic Immunity by Promoting Neutrophil Extracellular Trap Formation via the NFATc1/CXCL8 Axis

    Year:
    2024
    Journal Name:
    Advanced science (Weinheim, Baden-Württemberg, Germany)
    PubMed ID:
    39269257

  • Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion

    Species:
    Human
    Sample Type:
    Caco-2 cell
    Year:
    2023
    Journal Name:
    Food Research International

  • Lipoxin A4 promotes antibiotic and monocyte bacterial killing in established Pseudomonas aeruginosa biofilm formed under hydrodynamic conditions

    Species:
    Human
    Sample Type:
    THP-1 monocytes
    Year:
    2023
    Journal Name:
    FASEB JOURNAL

  • Conditioned Media from Head and Neck Cancer Cell Lines and Serum Samples from Head and Neck Cancer Patients Drive Catabolic Pathways in Cultured Muscle Cells

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Cancers

  • Dietary Fatty Acids Contribute to Maintaining the Balance between Pro-Inflammatory and Anti-Inflammatory Responses during Pregnancy

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Nutrients

  • Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Cancer Cell International

  • MiR-95-3p/EPM2A/MMP2 contributes to the pathogenesis of severe preeclampsia through the regulation of trophoblast biological behaviour

    Species:
    Human
    Sample Type:
    HTR-8/SVneo cell, JAR cell
    Year:
    2023
    Journal Name:
    Archives of Biochemistry and Biophysics

  • The uncharacterized transcript KIAA0930 confers a cachexic phenotype on cancer cells

    Species:
    Human
    Sample Type:
    Capan-2 cell, Mia PaCa-2 cell, 786-O cell, Caki-1 cell, PANC-1 cell, HCT 116 cell, HT29 cell, MKN45 cell
    Year:
    2023
    Journal Name:
    Oncotarget

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