This IL2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL2 protein in Serum, Cell supernatant, Plasma. It contains recombinant Human IL2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: Interleukin-2, also known as a T-cell growth factor, TCGF, Aldesleukin, and IL2, is a secreted protein that belongs to the IL-2 family. Interleukin-2/IL-2 was the first interleukin molecule to be discovered. Interleukin-2/IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2/IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2/IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells).Interleukin-2/IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2/IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2/IL-2 and the expression of IL-2 receptors IL-2R. The IL-2/IL-2R interaction then stimulates the growth, differentiation, and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2/IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2/IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2/IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2/IL-2 in HIV therapy is ineffective.

Smith KA, et al., 1980, J. Exp. Med.151 (6): 1551-6.
Smith KA, et al., 1980, Nature. 287 (5785): 853-5.
Taniguchi T, et al., 1983, Nature. 302 (5906): 305.
Cantrell DA, et al., 1984, Science. 224 (4655): 1312-6.
Smith KA, et al., 1988, Science. 240 (4856): 1169-76.
Wang X. et al., 2005, Science 310:1159-63.
Stauber D.J. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 2788-93.

Small volumes of IL2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8123955 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL2. The ELISA analytical biochemical technique of the MBS8123955 kit is based on IL2 antibody-IL2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).